5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (CAS 7240-90-6) is a vital chromogenic substrate used for the detection of β-galactosidase activity. Upon enzymatic cleavage, it yields a distinct, intense blue precipitate, facilitating clear visual identification. This compound is primarily employed in molecular biology for blue-white screening to identify recombinant bacterial colonies possessing the lac+ genotype.
- IUPAC
- 5-Bromo-4-chloro-3-indolyl β-D-galactoside,BCIG,X-Gal
- Synonyms
- 5-Bromo-4-chloro-3-indolyl β-D-galactosideBCIGX-Gal
Blue-White Screening
X-Gal is extensively used as a chromogenic substrate in molecular biology for the blue-white screening method. It enables the visual differentiation of bacterial colonies that have successfully incorporated a foreign DNA fragment.
Enzyme Activity Detection
This compound serves as a sensitive indicator for the presence and activity of the β-galactosidase enzyme. The formation of a deep blue colour provides a straightforward method for qualitative assessment of enzyme function.
Reporter Gene Assays
In research settings, X-Gal can be employed in reporter gene assays where the lacZ gene is used as a reporter, allowing for the monitoring of gene expression levels through observable colour changes.
| Molecular weight | 408.63 |
|---|---|
| Empirical formula | C14H15BrClNO6 |
| Assay | ≥98.0% (HPLC) |
| Optical activity | [α]20/D −62±2°, c = 1% in DMF/H2O 1:1 |
| Storage temperature | 2-8°C |
| Application | X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. |
| Suitability | suitable for identification of lac+ bacterial colonies |
| Availability | available only in Japan |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside used for?
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5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal) is primarily used as a chromogenic substrate for detecting β-galactosidase activity, notably in the blue-white screening method for identifying recombinant bacterial colonies.
What is the CAS number and formula for this compound?
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The CAS number is 7240-90-6 and its empirical formula (Hill notation) is C14H15BrClNO6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this compound with a purity of ≥98.0% (HPLC). TSS does not represent this product as USP, BP, or EP grade.
How should 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside be stored?
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It should be stored at 2-8°C to maintain its stability and efficacy.
Is this product available for export?
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Yes, Tech Serve Solutions is a global supplier and exporter of this chemical, available only in Japan.
How can I request a sample or quote?
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To request a sample or a quote, please contact our sales team through the website or by phone.
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