5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt
5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt (CAS: 114162-64-0) is a chromogenic substrate with a molecular weight of 521.79. It is specifically designed for the detection of β-glucuronidase (GUS) enzyme activity. This compound is widely utilised in molecular biology and glycobiology for gene expression studies and investigating post-translational modifications, leading to a visible colour change upon enzymatic cleavage.
- IUPAC
- X-GlcA,X-glucuronide CHA salt
- Synonyms
- X-GlcA,X-glucuronide CHA salt
Detection of β-Glucuronidase (GUS) Activity
Serves as a highly effective chromogenic substrate for detecting β-glucuronidase (GUS) enzyme activity. Its enzymatic cleavage results in a distinct colour change, making it invaluable for reporter gene assays.
Gene Expression Studies
Crucial for monitoring gene expression patterns in molecular biology research. The GUS reporter gene system, utilizing this substrate, allows for precise visualisation of gene activity in various biological systems.
Biochemical Research
Applicable in glycobiology and proteomics to study post-translational modifications. It aids in understanding enzyme function and metabolic pathways involving glucuronides.
| Molecular weight | 521.79 |
|---|---|
| Empirical formula | C14H13BrClNO7 · C6H13N |
| Assay | ≥98% |
| Form | tablet |
| Solubility | DMF: soluble |
| Storage temperature | −20°C |
| Application | Chromogenic substrate for β-glucuronidase (GUS) gene detection. |
| Suitability | suitable for β-galactosidase test |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Sterility | non-sterile |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt used for?
+
This compound is a chromogenic substrate primarily used for the detection of β-glucuronidase (GUS) enzyme activity, often in reporter gene assays for gene expression studies and for investigating post-translational modifications.
What is the CAS number and formula for this chemical?
+
The CAS number is 114162-64-0. The empirical formula (Hill notation) is C14H13BrClNO7 · C6H13N.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies this product with an assay of ≥98%. It is suitable for the β-galactosidase test and is not supplied as USP, BP, or EP grade.
What are the safety and handling considerations?
+
Appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter, should be used. The compound has a WGK Germany classification of 3, indicating it is hazardous to water.
How is this product packed, shipped, and exported?
+
This product is typically supplied non-sterile and stored at -20°C. Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, ensuring compliance with international shipping regulations.
How can I request a sample or a quote?
+
Please contact our customer service team or use the online enquiry form on the Tech Serve Solutions website to request a sample or a quotation for 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents
(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more
3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis
3-Methyl-1,5-pentanediol
Chemical Synthesis
3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis