5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt
5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt (CAS: 129541-41-9) is a chromogenic substrate essential for detecting β-glucuronidase (GUS) enzyme activity. With a molecular weight of 444.59 g/mol, this compound is widely utilised in molecular biology and glycobiology for gene expression studies and post-translational modification analysis. It serves as a key reagent for visualising enzyme activity in various biological systems.
- IUPAC
- BC-Indicator,X-GlcA,X-glucuronide
- Synonyms
- X-Gluc sodium saltBC-Glucuronide sodium salt5-Bromo-4-chloro-3-indolyl beta-D-glucuronide sodium salt
Chromogenic Detection of β-Glucuronidase (GUS)
This compound acts as a highly specific chromogenic substrate for the enzyme β-glucuronidase (GUS). Upon enzymatic cleavage, it liberates an indolyl derivative that dimerizes and oxidizes to form a distinct blue precipitate, enabling clear visualisation of enzyme activity.
Gene Expression Analysis
It is extensively employed in molecular biology for monitoring the activity of the GUS reporter gene in transgenic organisms. This allows researchers to assess gene expression patterns and regulatory elements in plants, bacteria, and other model systems.
Proteomics and Post-Translational Modifications
In proteomics research, it can be used to identify and study enzymes involved in glucuronidation, a significant post-translational modification. This aids in understanding protein function and cellular processes.
| Molecular weight | 444.59 |
|---|---|
| Empirical formula | C14H12BrClNNaO7 |
| Assay | ≥98% |
| Storage temperature | −20°C |
| Application | Chromogenic substrate for β-glucuronidase (GUS) gene detection. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt used for?
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5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt is primarily used as a chromogenic substrate for the detection of β-glucuronidase (GUS) enzyme activity, particularly in molecular biology for reporter gene assays and in glycobiology and proteomics.
What are the CAS number and formula for this compound?
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The CAS number is 129541-41-9, and the empirical formula is C14H12BrClNNaO7.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥98%. This product is intended for research and laboratory use only and is not represented as USP, EP, or BP grade.
How should 5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt be handled and stored?
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Handle with appropriate personal protective equipment, including eyeshields and gloves. A P1 (EN143) or N95 (US) respirator filter is recommended. Store at -20°C. The product has a WGK Germany rating of 3, indicating it is hazardous.
Is this product a controlled substance?
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The provided data does not indicate that this compound is a controlled substance.
How can I request a sample or quote?
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Please contact our sales team directly through our website or by phone to request a sample or obtain a quotation.
Does Tech Serve Solutions export this chemical?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals and laboratory reagents.
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