5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside

CAS 125328-84-9C14H15BrClNO5Biochemicals & Reagents

5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside (CAS: 125328-84-9) is a valuable biochemical reagent with the molecular formula C14H15BrClNO5 and a molecular weight of 392.63 g/mol. This compound serves as a chromogenic substrate, primarily utilised in enzymatic assays. It enables the detection and quantification of enzyme activity, particularly fucosidase, through the release of a coloured precipitate upon enzymatic cleavage. This makes it indispensable in various research and diagnostic applications within molecular biology and glycobiology.

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5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside (C14H15BrClNO5) — chemical structure, CAS 125328-84-9; Life Science, fine chemical supplied by Tech Serve Solutions

ActivityBiochemicals and ReagentsChromogenicEnzyme SubstratesEnzymesInhibitorsand SubstratesFluorogenicFucosideGlycobiology

01 /Applications

Enzyme Activity Detection

5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside acts as a sensitive chromogenic substrate for detecting fucosidase activity. Enzymatic hydrolysis releases a coloured indigo derivative, facilitating visual or spectrophotometric assessment of enzyme presence and quantity.

Glycobiology Research

This compound is instrumental in studying carbohydrate metabolism and glycoconjugate structures. It aids researchers in investigating enzymes involved in the breakdown or modification of fucose-containing glycans within biological systems.

Proteomics and Post-Translational Modifications

In proteomics, it can be used to identify enzymes responsible for specific glycosylation or deglycosylation events, offering insights into post-translational modifications that influence protein function and behaviour.

Diagnostic Assays

Its chromogenic properties make it suitable for developing diagnostic tests where the detection of fucosidase activity is clinically relevant, for example, in certain inherited metabolic disorders.

02 /Properties

Molecular weight	392.63
Empirical formula	C14H15BrClNO5
Assay	≥98%
Storage temperature	−20°C

03 /Identifiers & registry

CAS number

125328-84-9

MDL number

MFCD00063688

04 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

05 /Frequently asked questions

What is 5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside used for?

This compound is primarily used as a chromogenic substrate for the detection and assay of fucosidase enzyme activity, particularly in biochemical research, glycobiology, and diagnostic applications.

What is the CAS number and molecular formula?

The CAS number for 5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside is 125328-84-9. Its empirical formula is C14H15BrClNO5.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies 5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside with an assay of ≥98%. TSS does not represent this product as USP, BP, EP, or pharmacopoeia grade.

What are the safety and handling recommendations?

According to the supplied data, this compound has a WGK Germany rating of 3, indicating it is hazardous to water. Appropriate laboratory safety precautions should be taken during handling and storage.

How is this product shipped and exported?

Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals and reagents, adhering to all relevant shipping and export regulations for chemical products.

How can I request a sample or quote?

To request a sample or a quote for 5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside, please contact the Tech Serve Solutions sales team through our website or by phone.