5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt
5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt (CAS 6769-80-8) is a chromogenic substrate for alkaline phosphatase. This biochemical reagent is recognised for its ability to produce a distinct magenta precipitate upon enzymatic hydrolysis. With a molecular weight of 433.62, it is primarily employed in various biochemical and molecular biology assays, particularly for the detection and quantification of alkaline phosphatase activity. Its reliable performance makes it a valuable tool in research and diagnostic applications.
- IUPAC
- Magenta™ phosphate
Alkaline Phosphatase Detection
This compound serves as a key chromogenic substrate for the detection of alkaline phosphatase (AP) enzyme activity. Upon hydrolysis by AP, it yields a soluble magenta product, enabling the visual identification and quantification of enzyme presence.
Biochemical Assays
Utilised in a range of biochemical research settings to study enzyme kinetics and monitor biological processes where alkaline phosphatase plays a role. It is essential for qualitative and quantitative analyses.
Molecular Biology Applications
Frequently used in molecular biology for applications such as western blotting, immunohistochemistry, and in situ hybridisation, where AP-conjugated antibodies are employed for signal detection.
Chromogenic Detection Systems
Forms the basis of sensitive chromogenic detection systems, allowing for the precise localisation and measurement of AP activity in various biological samples and experimental setups.
| Molecular weight | 433.62 |
|---|---|
| Empirical formula | C8H6BrClNO4P · C7H9N |
| Assay | ~97% (HPLC) |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt used for?
+
5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt is primarily used as a chromogenic substrate for the detection of alkaline phosphatase (AP) activity in biochemical and molecular biology assays. It produces a magenta colour upon enzymatic hydrolysis.
What are the CAS number and molecular formula for this product?
+
The CAS number is 6769-80-8. The empirical formula is C8H6BrClNO4P · C7H9N.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies this product with an assay of approximately 97% (HPLC). We do not represent this product as USP, BP, EP, or pharmacopoeia grade.
How should this chemical be handled and stored?
+
Handle with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Store at -20°C to maintain stability.
Is this chemical a controlled substance in Germany?
+
This chemical is classified under WGK Germany 3.
How are Tech Serve Solutions products packaged and exported?
+
Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, pharmaceutical intermediates, and laboratory reagents. Products are carefully packaged to ensure integrity during transit and are exported worldwide.
How can I request a sample or quote for this product?
+
Please contact our sales team via the website or direct enquiry channels to request a sample or a quotation for 5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents
(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more
3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis
3-Methyl-1,5-pentanediol
Chemical Synthesis
3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis