5-Bromo-6-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt
5-Bromo-6-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt (CAS 144110-43-0) is a chromogenic substrate for beta-glucuronidase enzymes. Upon enzymatic cleavage, it yields a magenta colour, facilitating the visual identification of GUS-positive bacterial colonies. This biochemical reagent is supplied with an assay of ≥98% and is stored at -20°C. Its primary application lies in molecular biology and cloning, enabling researchers to detect enzyme activity in various biological systems.
- IUPAC
- Magenta-GlcA,Magenta™ glucuronide
- Synonyms
- Magenta-GlcAMagenta™ glucuronide5-Bromo-6-chloro-3-indolyl beta-D-glucuronide, cyclohexylammonium salt
Chromogenic Detection of β-Glucuronidase Activity
This compound serves as a highly effective chromogenic substrate for the enzyme β-glucuronidase. Its enzymatic hydrolysis by GUS releases a coloured product, indicated by a magenta hue, which is crucial for the visual screening of bacterial colonies expressing GUS activity.
Molecular Biology and Cloning Applications
In molecular biology and cloning workflows, Magenta-GlcA is used as a reporter substrate. It aids in identifying successful gene insertions or expression in genetically modified organisms, particularly in microbial systems.
Biochemical Research
As a specialized biochemical reagent, it is employed in various research settings to study glucuronidase enzyme kinetics, specificity, and activity in different biological matrices.
| Molecular weight | 521.79 |
|---|---|
| Empirical formula | C14H13BrClNO7 · C6H13N |
| Assay | ≥98% |
| Storage temperature | −20°C |
| Application | Chromogenic substrate for β-glucuronidase; produces a magenta color in GUS+ bacterial colonies. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Bromo-6-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt used for?
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This compound is primarily used as a chromogenic substrate for the detection of β-glucuronidase (GUS) enzyme activity, producing a magenta colour that is valuable for identifying GUS-positive bacterial colonies in molecular biology and cloning applications.
What is the CAS number and formula for this product?
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The CAS number is 144110-43-0. The empirical formula is C14H13BrClNO7 · C6H13N.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with a purity of ≥98%. We do not represent this product as USP, BP, EP, or any other pharmacopoeial grade unless explicitly stated in the specifications.
What are the safety and handling precautions for this chemical?
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This chemical requires appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The WGK Germany classification is 3, indicating a high hazard.
How is this product packed, shipped, and exported?
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We ensure secure and compliant packing for global export, adhering to all relevant shipping regulations for fine chemicals. Specific packaging details can be provided upon request.
How can I request a sample or a quote?
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Please contact our sales department via the website or by phone to request a sample or obtain a quotation for 5-Bromo-6-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt.
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