5(6)-Carboxyfluorescein diacetate
5(6)-Carboxyfluorescein diacetate (CAS: 124387-19-5, Formula: C25H16O9, Molecular Weight: 460.39) is a high-purity fluorescent probe supplied by Tech Serve Solutions. With an assay of ≥90.0% (HPLC), it is specifically suited for fluorescence applications. This compound is a valuable tool for cell staining and analysis in various research settings, particularly in flow cytometry.
- IUPAC
- 5(6)-Carboxy-di-O-acetylfluorescein
- Synonyms
- 5(6)-Carboxy-di-O-acetylfluorescein
Cell Staining and Viability Assays
5(6)-Carboxyfluorescein diacetate is employed as a fluorescent probe to stain cells, enabling the assessment of cell viability and function. Its ability to cross cell membranes and subsequent hydrolysis by intracellular esterases make it useful for tracking cellular processes.
Flow Cytometry Analysis
This compound is suitable for fluorescence-based analysis in flow cytometry. The resulting fluorescence, with excitation at 492 nm and emission at 517 nm in Tris pH 8.0, allows for the differentiation and quantification of cell populations.
Esterase Activity Detection
The diacetate groups are cleaved by intracellular esterases, releasing the fluorescent carboxyfluorescein. This property makes it a sensitive indicator for detecting and quantifying esterase activity within cells.
| Molecular weight | 460.39 |
|---|---|
| Empirical formula | C25H16O9 |
| Assay | ≥90.0% (HPLC) |
| Solubility | DMSO: soluble |
| Storage temperature | 2-8°C |
| Application | 5(6)-Carboxyfluorescein diacetate (CFDA) is a fluorescent probe used to stain cells for analysis in applications such as flow cytometry. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5(6)-Carboxyfluorescein diacetate used for?
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5(6)-Carboxyfluorescein diacetate is a fluorescent probe primarily used for cell staining and analysis in applications such as flow cytometry, and for detecting intracellular esterase activity.
What is the CAS number and molecular formula for 5(6)-Carboxyfluorescein diacetate?
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The CAS number is 124387-19-5 and the molecular formula is C25H16O9.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5(6)-Carboxyfluorescein diacetate with an assay of ≥90.0% (HPLC). We do not represent this product as USP, BP, or EP grade.
How should 5(6)-Carboxyfluorescein diacetate be handled and stored?
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Handle with appropriate personal protective equipment including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter may be required. Store at 2-8°C.
Is 5(6)-Carboxyfluorescein diacetate a controlled substance?
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The provided safety data indicates a WGK Germany classification of 3, but does not list it as a controlled substance.
How is this product shipped?
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This product is stored at 2-8°C and is available for export globally by Tech Serve Solutions.
How can I request a quote or sample?
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Please contact Tech Serve Solutions directly via our website or sales department to request a quote or sample of 5(6)-Carboxyfluorescein diacetate.
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