5(6)-Carboxyfluorescein diacetate N-succinimidyl ester
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CAS: 150347-59-4; C29H19NO11) is a fluorescein derivative, a bioconjugation reagent ideal for long-term cell tracing applications. Upon intracellular deacetylation by esterases, it generates a highly fluorescent green-emitting product, enabling detailed analysis of cellular processes. This compound is primarily used in cell culture and viability assays.
- IUPAC
- CFSE
Long-Term Cell Tracing
As an amine-reactive label, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is extensively used for long-term monitoring of cell populations. Its conversion to a fluorescent product within cells allows for tracking cell division and fate over extended periods.
Cell Viability Assays
This compound serves as a valuable reagent in cell viability assessments. The resulting green fluorescence provides a quantifiable measure to evaluate cell health and responses to various treatments or experimental conditions.
Fluorescence Microscopy and Imaging
The green fluorescence emitted after deacetylation makes it highly suitable for fluorescence microscopy. Researchers can visualise and quantify cellular distribution and proliferation in fixed or live cell imaging experiments.
| Molecular weight | 557.46 |
|---|---|
| Empirical formula | C29H19NO11 |
| Assay | ≥90% (HPLC) |
| Solubility | DMF: solubleDMSO: soluble |
| Storage temperature | −20°C |
| Application | Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation. |
| Suitability | suitable for fluorescence |
| Melting point | 152-154 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester used for?
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It is primarily used as an amine-reactive label for long-term tracing of cells, forming green fluorescent conjugates upon deacetylation. It is suitable for cell culture, viability assays, and fluorescence imaging.
What are the CAS number and molecular formula?
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The CAS number is 150347-59-4 and the molecular formula is C29H19NO11.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester with a purity of ≥90% (HPLC). TSS does not represent this product as USP, BP, or EP grade.
How should 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester be handled?
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Appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or P1 (EN143) respirator filter, should be used when handling this chemical. It has a WGK rating of 3 in Germany.
How is this chemical packed, shipped, and exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals. Specific details regarding packing and shipping for international export can be provided upon request.
How can I request a sample or quote for 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester?
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Please contact our sales team directly via our website or by phone to request a sample or a detailed quotation for 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester.
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