5(6)-Carboxy-X-rhodamine N-succinimidyl ester
5(6)-Carboxy-X-rhodamine N-succinimidyl ester (CAS: 114616-32-9; Formula: C37H33N3O7) is a fluorescent labeling reagent. This compound enables the covalent attachment of the 5(6)-carboxy-X-rhodamine fluorophore to target molecules. It is primarily utilised in biochemical research for applications such as fluorescence energy transfer (FRET) studies and for labelling biomolecules for sensitive detection.

Fluorescent Labelling
5(6)-Carboxy-X-rhodamine N-succinimidyl ester serves as a reactive dye for covalently labelling biomolecules, such as proteins and nucleic acids, with a rhodamine fluorophore.
Fluorescence Energy Transfer (FRET)
Molecules labelled with this rhodamine derivative can be employed in FRET-based assays to study molecular interactions and conformational changes.
Biochemical Research
This reagent is valuable for researchers in biochemistry and molecular biology investigating cellular processes and molecular dynamics using fluorescent detection methods.
| Empirical formula | C37H33N3O7 |
|---|---|
| Solubility | DMF: solubleacetonitrile: soluble |
| Storage temperature | −20°C |
| Application | 5(6)-Carboxy-X-rhodamine N-succinimidyl ester may used as a labeling reagent to attach the fluorophore 5(6)-carboxy-X-rhodamine to target molecules such as nucleic acids. 5(6)-Carboxy-X-rhodamine labeled molecules may be used in fluorescence energy transfer (FRET) applications. |
| Suitability | suitable for fluorescence |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 575 nm; λem 605 nm in 0.1 M phosphate pH 7.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5(6)-Carboxy-X-rhodamine N-succinimidyl ester used for?
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This compound is primarily used as a fluorescent labeling reagent for attaching the 5(6)-carboxy-X-rhodamine fluorophore to target molecules like nucleic acids, enabling applications such as fluorescence energy transfer (FRET).
What is the CAS number and formula for 5(6)-Carboxy-X-rhodamine N-succinimidyl ester?
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The CAS number is 114616-32-9, and the empirical formula is C37H33N3O7.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product as a BioReagent. We do not represent it as USP, BP, or EP grade. Please refer to the Certificate of Analysis for specific purity details.
How should 5(6)-Carboxy-X-rhodamine N-succinimidyl ester be handled safely?
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This compound is a Warning substance, classified with H315 (Causes skin irritation), H319 (Causes serious eye irritation), and H335 (May cause respiratory irritation). Use with appropriate personal protective equipment including a dust mask (type N95 US), eyeshields, and gloves in a well-ventilated area. Avoid breathing dust.
How is this chemical shipped and exported?
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As a specialist global supplier, Tech Serve Solutions offers comprehensive export services for 5(6)-Carboxy-X-rhodamine N-succinimidyl ester, ensuring compliant and secure packaging and transportation.
How can I request a sample or quote?
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To request a sample or a quotation for 5(6)-Carboxy-X-rhodamine N-succinimidyl ester, please contact our sales team through the website or by phone.
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