5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

CAS 127770-45-0C25H14Cl2O9Biochemicals & Reagents

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CAS 127770-45-0) is a biochemical reagent with the empirical formula C25H14Cl2O9 and a molecular weight of 529.28. This fluorescent probe is primarily utilised for studying the distribution and mechanisms of multidrug resistance-associated protein 2 (Mrp2). Its fluorescence properties make it suitable for various detection applications in biochemical research and analysis, often employed in microscopy and flow cytometry.

Synonyms: 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate

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5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (C25H14Cl2O9) — chemical structure, CAS 127770-45-0; Life Science, fine chemical supplied by Tech Serve Solutions

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01 /Applications

Fluorescent Substrate for Mrp2 Studies

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate serves as a critical fluorescent substrate for investigating the functionality and cellular distribution of multidrug resistance-associated protein 2 (Mrp2). It allows researchers to visualise and quantify Mrp2 activity in biological systems.

Biochemical Detection and Analysis

This compound is employed as a fluorescent probe in various biochemical assays. Its luminescence characteristics enable sensitive detection in research settings, particularly within flow cytometry and microscopy applications.

Enzyme Activity Assays

As a fluorescent enzyme substrate, it can be used to monitor enzymatic reactions. The cleavage of the acetate groups by esterases leads to the release of a fluorescent signal, indicating enzyme activity.

02 /Properties

Molecular weight	529.28
Empirical formula	C25H14Cl2O9
Assay	≥85% (HPCE)
Solubility	DMF: solubleDMSO: solublemethanol: soluble
Storage temperature	−20°C
Application	5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CDCF) is used as a fluorescent substrate/probe to study the distribution and mechanism of action of multidrug resistance-associated protein 2 (Mrp2).
Suitability	suitable for fluorescence
Melting point	210 °C (dec.)(lit.)

03 /Safety & handling

GHS hazard pictogram: Harmful / irritant (GHS07) — Harmful / irritant

Warning

Hazard statements

H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust, fume, gas or vapours
P305IF IN EYES

Protective equipment	dust mask type N95 (US), Eyeshields, Gloves
Water hazard class (WGK, DE)	3
Hazard codes (EU)	Xi
Risk statements (R)	36/37/38
Safety statements (S)	26

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

127770-45-0

MDL number

MFCD08459585

Beilstein registry

7505106

05 /Additional specifications

Product Line	BioReagent
Fluorescence	λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate used for?

This compound is used as a fluorescent substrate and probe to study the distribution and mechanism of action of multidrug resistance-associated protein 2 (Mrp2), and for general fluorescent detection in biochemical assays.

What is the CAS number and molecular formula for this product?

The CAS number is 127770-45-0 and the empirical formula is C25H14Cl2O9.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies this product with an assay of ≥85% (HPCE). TSS does not represent this product as USP, BP, or EP grade.

What are the safety considerations for handling 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate?

This compound carries a GHS07 symbol and is classified as a Warning. It is associated with hazard statements H315 (Causes skin irritation), H319 (Causes serious eye irritation), and H335 (May cause respiratory irritation). Appropriate personal protective equipment, including a dust mask (type N95), eyeshields, and gloves, should be worn. Handle in a well-ventilated area.

How is this chemical packed, shipped, and exported?

Tech Serve Solutions, established in 1998, is a specialist global supplier and exporter of fine chemicals. Products are carefully packed according to industry standards and regulations for safe domestic and international shipment.

How can I request a sample or a quote for 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate?

To request a sample or obtain a quotation, please contact our sales department through the website or by phone. Our team will be pleased to assist you with your requirements.