5(6)-Carboxyfluorescein N-hydroxysuccinimide ester
5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (CAS: 117548-22-8; C25H15NO9) is a highly fluorescent labelling reagent. It reacts with primary and secondary amines, making it ideal for labelling proteins and other biomolecules. Its distinct excitation (492 nm) and emission (517 nm) spectra in DMF facilitate sensitive detection in various biological applications, including microscopy and flow cytometry.
- IUPAC
- 5(6)-FAM SE,FLUOS
Fluorescent Labelling of Biomolecules
This succinimidyl ester derivative of carboxyfluorescein is specifically designed to react with primary and secondary amine groups present in proteins and other biological macromolecules. This allows for the covalent attachment of the fluorescent carboxyfluorescein tag.
Bioconjugation Chemistry
As a reactive fluorescent probe, it is widely employed in bioconjugation strategies for creating labelled antibodies, peptides, and other biomolecules. The resulting conjugates can be used in various immunoassays and diagnostic applications.
Cellular Imaging and Analysis
The bright fluorescence of carboxyfluorescein derivatives makes them suitable for intracellular labelling and tracking, as well as for use in flow cytometry and fluorescence microscopy experiments to visualise cellular structures or processes.
| Molecular weight | 473.39 |
|---|---|
| Empirical formula | C25H15NO9 |
| Assay | ≥90% (HPLC) |
| Solubility | DMF: solubleDMSO: soluble |
| Storage temperature | −20°C |
| Application | Fluorescent label for primary and secondary amine groups on proteins and other biomolecules. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 492 nm; λem 517 nm in DMF |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester used for?
+
5(6)-Carboxyfluorescein N-hydroxysuccinimide ester is primarily used as a fluorescent label for proteins and other biomolecules containing primary and secondary amine groups, enabling their detection and visualisation in biological studies.
What are the CAS number and formula for 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester?
+
The CAS number is 117548-22-8 and the empirical formula is C25H15NO9.
What grade and purity does Tech Serve Solutions supply for 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester?
+
Tech Serve Solutions supplies 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester with an assay of ≥90% (HPLC). TSS does not represent this product as USP, BP, or EP grade.
How should 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester be handled safely?
+
Handling requires appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. It has a WGK Germany rating of 3, indicating it is hazardous.
How is 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester packed and shipped?
+
This product is typically stored at -20°C. Consult our shipping policies for details on export and delivery of fine chemicals.
How can I request a quote or sample for 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester?
+
Please contact our sales department through the website or by phone to request a quotation or a sample for 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents
(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more
3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis
3-Methyl-1,5-pentanediol
Chemical Synthesis
3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis