4-Nitrophenyl acetate
4-Nitrophenyl acetate (CAS 830-03-5), with the molecular formula CH3CO2C6H4NO2 and a molar mass of 181.15 g/mol, is a chemical compound recognised for its role in biochemical research and chemical synthesis. It serves as a substrate in enzymatic assays, particularly for esterases and acetylcholinesterase, due to its chromogenic hydrolysis product. This compound is a valuable reagent for laboratory research, analytical standards, and organic synthesis, finding utility in various scientific disciplines.
- IUPAC
- Acetic acid 4-nitrophenyl ester

Enzyme Substrate
4-Nitrophenyl acetate is frequently used as a chromogenic substrate for assays involving esterase and acetylcholinesterase activity. Hydrolysis releases 4-nitrophenol, which can be quantified spectrophotometrically.
Analytical Standard
This compound functions as an analytical standard in chromatography and other analytical techniques. Its well-defined properties make it suitable for calibration and method development in research laboratories.
Chemical Synthesis Reagent
As an organic building block, 4-Nitrophenyl acetate is employed in various chemical synthesis pathways. It can be used to introduce the 4-nitrophenoxy group or as a precursor in the development of more complex molecules.
Biochemical Research
It is utilized in studies concerning enzyme kinetics and biochemical pathways, particularly those involving ester hydrolysis and enzyme inhibition.
| Molecular weight | 181.15 |
|---|---|
| Linear formula | CH3CO2C6H4NO2 |
| Assay | ≥99.0% (GC) |
| Storage temperature | −20°C |
| Melting point | 75-77 °C(lit.)77-79 °C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Gene Information | human ... PON1(5444) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl acetate used for?
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4-Nitrophenyl acetate is primarily used as an enzyme substrate, particularly for esterases and acetylcholinesterase, and as an analytical standard in chemical research and synthesis.
What is the CAS number and molecular formula for 4-Nitrophenyl acetate?
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The CAS number for 4-Nitrophenyl acetate is 830-03-5, and its molecular formula is CH3CO2C6H4NO2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitrophenyl acetate with an assay of ≥99.0% (GC). This is a research-grade chemical and is not represented as USP, BP, EP, or any other pharmacopoeia standard.
What are the main safety concerns when handling 4-Nitrophenyl acetate?
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Handling 4-Nitrophenyl acetate requires appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. It is classified with WGK Germany 3, indicating a high hazard to water.
How is 4-Nitrophenyl acetate packed, shipped, and exported?
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4-Nitrophenyl acetate is packed and shipped according to relevant regulations for chemical compounds. Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals.
How can I request a sample or quote for 4-Nitrophenyl acetate?
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To request a sample or a quote for 4-Nitrophenyl acetate, please contact our sales department through the TSS website or by phone.
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