Tetrazolium Blue Chloride

CAS 1871-22-3C40H32Cl2N8O2Biochemicals & Reagents

Tetrazolium Blue Chloride (CAS: 1871-22-3) is a chemical compound with the formula C40H32Cl2N8O2 and a molecular weight of 727.64 g/mol. Commonly known as BTC, it functions as a chromogenic agent and is primarily utilized as an indicator in microbiological applications and for assessing cellular viability. Its ability to form coloured formazan products upon reduction makes it valuable in various biochemical assays.

IUPAC: 3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride,3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride),BTC,Blue Tetrazolium chloride,TZ
Synonyms: BTCBlue Tetrazolium chloride3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride)TZ

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Tetrazolium Blue Chloride (C40H32Cl2N8O2) — chemical structure, CAS 1871-22-3; Life Science, fine chemical supplied by Tech Serve Solutions

Alkaline PhosphataseBiochemicals and ReagentsCytokinin dehydrogenaseEnzyme SubstratesEnzymesInhibitorsand SubstratesFuroyl-CoA dehydrogenaseGlucose OxidaseLactic Dehydrogenase

01 /Applications

Microbiological Indicator

Tetrazolium Blue Chloride serves as a key indicator in microbiological media, aiding in the assessment of bacterial growth and metabolic activity. Its reduction by microbial enzymes produces a distinct colour change, facilitating easy observation and quantification.

Cell Viability Assays

This compound is employed in cell-based assays to determine cellular metabolic activity, often correlating with cell viability. It is reduced by dehydrogenases within viable cells to form a coloured formazan precipitate.

Enzyme Substrate

As an enzyme substrate, Tetrazolium Blue Chloride is used in conjunction with specific enzymes like alkaline phosphatase and dehydrogenases. The enzymatic reaction leads to the formation of a coloured product, useful for enzyme activity studies.

02 /Properties

Molecular weight	727.64
Empirical formula	C40H32Cl2N8O2
Assay	≥90% (T)
Grade	for microbiologyindicator for germination
Melting point	255 °C (dec.)(lit.)

03 /Safety & handling

GHS hazard pictogram: Health hazard (GHS08) — Health hazard

Danger

Hazard statements

H350May cause cancer

Precautionary statements

P201Obtain special instructions before use
P308IF exposed or concerned

Protective equipment	Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
Water hazard class (WGK, DE)	3
Hazard codes (EU)	T
Risk statements (R)	45
Safety statements (S)	53-45

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

1871-22-3

MDL number

MFCD00040933

Beilstein registry

3894077

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is Tetrazolium Blue Chloride used for?

Tetrazolium Blue Chloride is primarily used as an indicator in microbiological applications and for assessing cellular viability, where it is reduced by cellular enzymes to form a coloured product.

What are the CAS number and formula for Tetrazolium Blue Chloride?

The CAS number for Tetrazolium Blue Chloride is 1871-22-3, and its molecular formula is C40H32Cl2N8O2.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Tetrazolium Blue Chloride with an assay of ≥90% (T) and a grade suitable for microbiology and as an indicator for germination. We do not represent this product as USP, BP, EP, or for pharmaceutical use unless explicitly stated in the product data.

What are the safety considerations for handling Tetrazolium Blue Chloride?

Tetrazolium Blue Chloride is classified as a Danger (GHS08) substance, with hazard statement H350 (May cause cancer). Appropriate personal protective equipment, including eyeshields, gloves, and specific respirator types (N100 or P3), is required for safe handling. Always consult the Safety Data Sheet before use.

How is Tetrazolium Blue Chloride packed and shipped?

Tech Serve Solutions exports fine chemicals globally. Specific packing and shipping details for Tetrazolium Blue Chloride are available upon request, adhering to international shipping regulations for hazardous materials.

How can I request a sample or quote for Tetrazolium Blue Chloride?

To request a sample or a quote for Tetrazolium Blue Chloride, please contact our sales team through the website or by calling us directly. Provide the product name and CAS number for prompt service.