Tetranitroblue tetrazolium chloride

CAS 1184-43-6C40H28Cl2N12O10Biochemicals & Reagents

Tetranitroblue tetrazolium chloride (CAS: 1184-43-6; Formula: C40H28Cl2N12O10; Molar Mass: 907.63 g/mol) is a tetrazolium salt primarily utilised as a chromogenic substrate in biochemical assays. It is known for its role in detecting enzymatic activity, particularly in histochemistry and immunology, where it forms a deep blue diformazan precipitate upon reduction. This compound is supplied by TSS for research and development purposes.

IUPAC: 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2,5-bis(p-nitrophenyl)-2H-tetrazolium chloride],TNBT,Tetranitrotetrazolium blue chloride
Synonyms: 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2,5-bis(p-nitrophenyl)-2H-tetrazolium chloride],TNBT,Tetranitrotetrazolium blue chloride

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Tetranitroblue tetrazolium chloride (C40H28Cl2N12O10) — chemical structure, CAS 1184-43-6; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsEnzyme SubstratesEnzymesInhibitorsand SubstratesGlucose OxidaseLactic DehydrogenasePeroxidase SubstratesSubstrates by Enzyme

01 /Applications

Enzyme Substrate

Tetranitroblue tetrazolium chloride serves as a sensitive chromogenic substrate for various oxidoreductase enzymes, including dehydrogenases and peroxidases. Its reduction yields a coloured formazan product, facilitating the quantitative or qualitative detection of enzyme activity in biological samples.

Histochemistry

This reagent is widely employed in histochemical staining techniques to visualise enzyme activity within tissue sections. The insoluble formazan precipitate forms at the site of enzymatic action, allowing for detailed localisation of specific enzyme functions.

Immunology and Cell Biology

Tetranitroblue tetrazolium chloride can be used in certain immunological assays and cell-based studies. It has been applied to detect cellular reduction processes and as an indicator in specific enzyme-linked assays, contributing to the study of cellular metabolism and function.

02 /Properties

Molecular weight	907.63
Empirical formula	C40H28Cl2N12O10
Assay	≥90%
Loss on drying	≤25% loss on drying
Storage temperature	2-8°C
Melting point	~170 °C (dec.)

03 /Safety & handling

GHS hazard pictogram: Health hazard (GHS08) — Health hazard

Danger

Hazard statements

H350May cause cancer

Precautionary statements

P201Obtain special instructions before use
P308IF exposed or concerned

Protective equipment	Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
Water hazard class (WGK, DE)	3
Hazard codes (EU)	T
Risk statements (R)	45
Safety statements (S)	53
Ignition residue	≤0.1%

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

1184-43-6

MDL number

MFCD00036338

Beilstein registry

3897475

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is Tetranitroblue tetrazolium chloride used for?

Tetranitroblue tetrazolium chloride is primarily used as a chromogenic substrate in biochemical assays, histochemistry, and immunology to detect the activity of oxidoreductase enzymes by forming a coloured formazan precipitate.

What are the CAS number and formula for Tetranitroblue tetrazolium chloride?

The CAS number is 1184-43-6, and the empirical formula is C40H28Cl2N12O10.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Tetranitroblue tetrazolium chloride with an assay of ≥90%. We do not represent this product as USP, BP, or EP grade.

How should Tetranitroblue tetrazolium chloride be handled?

Handling Tetranitroblue tetrazolium chloride requires caution due to its potential carcinogenic properties (H350). Use appropriate personal protective equipment including eyeshields, gloves, and a suitable respirator (type N100 US or P3 EN143). Store at 2-8°C and avoid exposure; refer to the Safety Data Sheet for full details.

Is Tetranitroblue tetrazolium chloride a controlled substance?

The provided data does not indicate that Tetranitroblue tetrazolium chloride is a controlled substance.

How is Tetranitroblue tetrazolium chloride packed and shipped?

Tetranitroblue tetrazolium chloride is typically packed in accordance with regulatory standards for hazardous chemicals and exported globally by TSS. Specific packaging details can be confirmed upon request.

How can I request a quote or sample?

To request a quote or sample of Tetranitroblue tetrazolium chloride, please contact the TSS sales team through our website or by phone, providing your specific requirements and shipping details.