Tetramethylrhodamine-5-isothiocyanate
Tetramethylrhodamine-5-isothiocyanate (CAS: 80724-19-2), with the molecular formula C25H21N3O3S, is a fluorescent labeling reagent. It is primarily utilised in biochemical and cell biology research for conjugation to biomolecules, enabling their detection and tracking in various biological systems. Its fluorescent properties make it a valuable tool for microscopy and other imaging techniques. Supplied by Tech Serve Solutions, it is essential for advanced biological investigations.
- IUPAC
- 5-TRITC,Xanthylium

Fluorescent Labeling of Biomolecules
Tetramethylrhodamine-5-isothiocyanate reacts with primary amines on proteins and other biomolecules to create stable fluorescent conjugates. These labelled molecules can then be used to study cellular processes, protein localisation, and interactions.
Cell Biology Research
This reagent is instrumental in cell biology for visualising and tracing cellular components or pathways. Its fluorescence allows for high-sensitivity detection in immunofluorescence assays and flow cytometry.
Biochemical Assays
As a fluorescent probe, it can be incorporated into biochemical assays to monitor enzymatic activity or binding events. The resulting fluorescent signal provides quantitative data on biological reactions.
| Molecular weight | 443.52 |
|---|---|
| Empirical formula | C25H21N3O3S |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Shipped In | dry ice |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Tetramethylrhodamine-5-isothiocyanate used for?
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Tetramethylrhodamine-5-isothiocyanate is used as a fluorescent label for biomolecules, particularly for conjugation with proteins and antibodies. It is valuable in cell biology, biochemical assays, and various imaging techniques.
What are the CAS number and molecular formula for Tetramethylrhodamine-5-isothiocyanate?
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The CAS number for Tetramethylrhodamine-5-isothiocyanate is 80724-19-2 and its molecular formula is C25H21N3O3S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Tetramethylrhodamine-5-isothiocyanate. The exact grade and purity are available on the product's Certificate of Analysis. TSS does not represent this product as USP, EP, BP, or any other pharmacopoeia grade.
How should Tetramethylrhodamine-5-isothiocyanate be handled safely?
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Handling Tetramethylrhodamine-5-isothiocyanate requires appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. Ensure adequate ventilation when handling. The product has a WGK Germany rating of 3, indicating it is hazardous.
How is Tetramethylrhodamine-5-isothiocyanate shipped and stored?
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Tetramethylrhodamine-5-isothiocyanate is shipped on dry ice and should be stored at -20°C to maintain its stability and efficacy.
How can I request a quote or sample for Tetramethylrhodamine-5-isothiocyanate?
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To request a quote or sample for Tetramethylrhodamine-5-isothiocyanate, please contact Tech Serve Solutions directly through our website or sales department, providing the product name and CAS number.
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