Stains-All
Stains-All (CAS 7423-31-6), with the empirical formula C30H27BrN2S2 and a molecular weight of 559.58, is a specialised dye used for differential staining in molecular biology. It effectively stains nucleic acids and proteins, with distinct colourations for RNA (bluish purple), DNA (blue), and proteins (red). Its high sensitivity allows for the detection of as little as 3 ng of DNA fragments on polyacrylamide gels. This reagent is supplied by Tech Serve Solutions for laboratory research purposes.
- IUPAC
- 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide,3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
Nucleic Acid Staining
Stains-All is employed to differentiate and visualise RNA (bluish purple) and DNA (blue) after electrophoresis on polyacrylamide gels. It is sensitive enough to detect very small quantities of DNA fragments.
Protein Staining
This stain provides a distinct red colouration for proteins, enabling their visualisation on gels following electrophoretic separation. It is useful in proteomic studies and general protein analysis.
Electrophoresis Analysis
Used in various electrophoresis techniques, including protein electrophoresis, Stains-All aids in the differential analysis of biomolecules after separation. The staining and destaining process requires controlled light exposure.
| Molecular weight | 559.58 |
|---|---|
| Empirical formula | C30H27BrN2S2 |
| Assay | ~95% |
| Storage temperature | 2-8°C |
| Application | Stains-all is suitable for differential staining of nucleic acids and proteins as follows; RNA (bluish purple), DNA (blue) and proteins (red). As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Stains-All used for?
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Stains-All is used for the differential staining of nucleic acids (RNA and DNA) and proteins following electrophoresis on polyacrylamide gels. RNA is visualised as bluish purple, DNA as blue, and proteins as red.
What is the CAS number and empirical formula for Stains-All?
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The CAS number for Stains-All is 7423-31-6, and its empirical formula is C30H27BrN2S2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Stains-All with an assay of approximately 95%. This product is intended for laboratory research purposes and is not represented as USP, BP, EP, or pharmacopoeia grade.
How should Stains-All be handled and stored?
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When handling Stains-All, personal protective equipment including eyeshields, gloves, and an appropriate respirator filter (type N95 or P1) should be used. The recommended storage temperature is between 2-8°C.
How is Stains-All packed, shipped, and exported?
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Tech Serve Solutions professionally packs, ships, and exports Stains-All globally, adhering to all relevant regulations for chemical transport. Specific packaging details are available upon request.
How can I request a quote or sample of Stains-All?
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To request a quote or a sample of Stains-All, please contact our sales team through the website or by phone. Our specialists will be pleased to assist you.
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