Ponceau S
Ponceau S (CAS: 6226-79-5, C22H12N4Na4O13S4) is a water-soluble azo dye with a molecular weight of 760.57 g/mol. It is primarily utilised in biochemical applications as a reversible stain for proteins in electrophoretic gels. Its vibrant colour and selective binding properties make it an effective tool for visualisation during molecular biology research and diagnostics.
- IUPAC
- 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt
Protein Staining in Electrophoresis
Ponceau S is widely employed as a reversible stain for detecting total protein on membranes (e.g., nitrocellulose, PVDF) following Western blotting or other protein transfer techniques. It allows for the visualisation of protein bands before proceeding to antibody incubations.
Biochemical Research Reagent
This azo dye serves as a valuable reagent in various biochemical assays. Its defined spectral properties, such as absorption maxima around 518-522 nm, contribute to its utility in quantitative or qualitative analytical methods.
Diagnostic Applications
In specific diagnostic protocols, Ponceau S can be used to confirm the presence and distribution of proteins on solid supports, aiding in the interpretation of experimental results in research settings.
| Molecular weight | 760.57 |
|---|---|
| Empirical formula | C22H12N4Na4O13S4 |
| Density | 1.01 g/mL at 20 °C |
| Refractive index | n20/D 1.338 |
| Solubility | H2O: soluble1 mg/mL |
| Concentration | ~0.1 % (w/v) Ponceau S in 5% acetic acid |
| Suitability | in accordance for electrophoresis test |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 27195 |
|---|---|
| Absorption | extinction/518-522 nm, deionized water ≥330000 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Ponceau S used for?
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Ponceau S is predominantly used as a reversible stain for proteins on membranes after electrophoresis, particularly in Western blotting, and as a general biochemical reagent for detection and visualisation.
What is the CAS number and formula for Ponceau S?
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The CAS number for Ponceau S is 6226-79-5, and its empirical formula is C22H12N4Na4O13S4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Ponceau S that is suitable for electrophoresis testing, with an extinction value of ≥330000 at 518-522 nm in deionised water. TSS does not represent this product as USP, BP, EP or other pharmacopoeial grades.
How should Ponceau S be handled safely?
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Handle Ponceau S with appropriate personal protective equipment, including a dust mask (N95 US type), eyeshields, and gloves. Avoid breathing dust and ensure adequate ventilation, as it may cause skin irritation, eye irritation, and respiratory irritation (H315, H319, H335).
How is Ponceau S packed and shipped?
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Ponceau S is carefully packed and exported by TSS in compliance with all relevant shipping regulations to ensure safe and secure delivery worldwide.
How can I request a sample or a quote for Ponceau S?
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To request a sample or a quote for Ponceau S, please contact our sales team through the website or by direct enquiry, providing your contact details and required quantity.
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