Neutral Red
Neutral Red (CAS: 553-24-2, Formula: C15H17ClN4, Molar Mass: 288.78 g/mol) is a vital chemical compound utilised primarily as a biological stain and a pH indicator. Its distinct colour change within a specific pH range makes it invaluable in laboratory settings for various analytical and cellular applications. This dye is also employed in preparing neutral red paper for qualitative testing and is suitable for cell culture media.
- IUPAC
- 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride,Basic Red 5,Toluylene red

Biological Staining
Neutral Red serves as a crucial biological stain, particularly in histology and cell biology. It is effectively used to stain cell components and for general histological staining purposes, aiding in microscopic examination and analysis.
pH Indication
This compound functions as a pH indicator with a transition range of 6.8 to 8.0, changing from red to yellow. This property makes it useful for titrations and monitoring pH in various chemical and biological systems.
Cell Culture
Neutral Red is suitable for cell culture applications. It can be used to assess cell viability and cellular functions, providing valuable insights in research and biotechnological processes.
Preparation of Indicator Paper
The chemical is employed in the preparation of neutral red paper. This specialised paper is used for rapid, qualitative assessment of pH levels in different samples.
| Molecular weight | 288.78 |
|---|---|
| Empirical formula | C15H17ClN4 |
| Concentration | 3.3 g/L in DPBS |
| Storage temperature | 2-8°C |
| Impurities tested | endotoxin, tested |
| Application | Useful as an indicator for preparing neutral red paper, and as a biological stain. |
| Suitability | suitable for cell culture |
| Melting point | 290 °C (dec.)(lit.) |
| Composition | Dye content, ≥90% |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 50040 |
|---|---|
| Sterility | sterile-filtered |
| Ph Range | 6.8 - 8.0, red to yellow |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Neutral Red used for?
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Neutral Red is primarily used as a biological stain in histology and cell biology, and as a pH indicator with a range of 6.8-8.0. It is also used for preparing neutral red paper and is suitable for cell culture.
What are the CAS number and formula for Neutral Red?
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The CAS number for Neutral Red is 553-24-2, and its molecular formula is C15H17ClN4.
What grade and purity does Tech Serve Solutions supply for Neutral Red?
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Tech Serve Solutions supplies Neutral Red with a dye content of ≥90%. It is sterile-filtered and suitable for cell culture. We do not represent this product as meeting any pharmacopoeial standards unless explicitly stated.
How should Neutral Red be handled and stored?
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Neutral Red should be handled with appropriate personal protective equipment, including eyeshields and gloves. A P1 (EN143) or N95 (US) respirator filter is recommended. It should be stored at 2-8°C.
Is Neutral Red a controlled substance?
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The provided data does not indicate that Neutral Red is a controlled substance.
How can I get a quote or sample for Neutral Red?
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Please contact our sales team through the website or by phone to request a quotation or to arrange for a sample of Neutral Red.
How is Neutral Red typically packaged and exported?
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Neutral Red is carefully packaged to ensure stability during transit and is available for global export by Tech Serve Solutions.
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