Naphthol AS-E phosphate
Naphthol AS-E phosphate (CAS: 18228-17-6) is a chemical compound with the molecular formula C17H13ClNO5P and a molecular weight of 377.72 g/mol. It functions as a chromogenic substrate for enzyme detection. Supplied by Tech Serve Solutions for laboratory and research applications, it is primarily utilised in biochemical assays and histochemical staining for the detection of phosphatase activity.
- IUPAC
- KG-501

Histochemical Staining
Naphthol AS-E phosphate is used as a substrate in histochemical techniques to visualise the activity of acid and alkaline phosphatases in tissue samples. The enzymatic hydrolysis releases naphthol, which then couples to form a coloured precipitate at the site of enzyme activity.
Biochemical Assays
In biochemical research, this compound serves as a substrate for quantifying acid and alkaline phosphatase activity in various biological samples. Its use allows for the spectrophotometric determination of enzyme kinetics and levels.
Enzyme Detection
It acts as a sensitive chromogenic substrate for the detection of specific enzyme activities within cellular or tissue environments. This application is crucial in diagnostics and fundamental research.
| Molecular weight | 377.72 |
|---|---|
| Empirical formula | C17H13ClNO5P |
| Grade | for histology |
| Storage temperature | 2-8°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Naphthol AS-E phosphate used for?
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Naphthol AS-E phosphate is used as a chromogenic substrate for the detection and quantification of acid and alkaline phosphatase activity in histochemical staining and biochemical assays.
What is the CAS number and formula for Naphthol AS-E phosphate?
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The CAS number is 18228-17-6, and the empirical formula is C17H13ClNO5P.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Naphthol AS-E phosphate for histology. TSS does not represent this product as USP, BP, or EP grade.
What are the safety precautions for handling Naphthol AS-E phosphate?
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Handle Naphthol AS-E phosphate with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. It is classified with WGK Germany 3.
How is Naphthol AS-E phosphate shipped and exported?
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Naphthol AS-E phosphate is stored at 2-8°C. Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals.
How can I request a sample or quote for Naphthol AS-E phosphate?
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Please contact our sales team via the website or directly to request a sample or a detailed quotation for Naphthol AS-E phosphate.
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