Naphthol AS-BI phosphate disodium salt hydrate
Naphthol AS-BI phosphate disodium salt hydrate (CAS: 207569-04-8) is a biochemical reagent utilized primarily as an enzyme substrate for detection and histochemical applications. With an empirical formula of C18H13BrNNa2O6P · xH2O and a molecular weight of 496.16 (anhydrous basis), this compound is characterized by its high purity (≥99.5%). It is soluble in water, making it convenient for various laboratory procedures requiring enzymatic activity monitoring.
- IUPAC
- 2-Naphthalenecarboxamide, 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)-, disodium salt hydrate
Enzyme Substrate
This disodium salt hydrate functions as a chromogenic substrate, particularly in assays involving phosphatases. Its enzymatic hydrolysis releases a naphthol derivative, which can then couple to form a coloured precipitate, enabling the detection and quantification of enzyme activity.
Histochemical Staining
The compound is employed in histochemistry for the visualization of enzyme activity within tissue sections. Its ability to form insoluble coloured products at the site of enzyme action aids in cellular and subcellular localization studies.
Diagnostic Reagent
As a sensitive detection reagent, it can be incorporated into diagnostic kits and assays for various biological markers where phosphatase activity is indicative of a specific condition or biological process.
| Molecular weight | 496.16 (anhydrous basis) |
|---|---|
| Empirical formula | C18H13BrNNa2O6P · xH2O |
| Assay | ≥99.5% |
| Solubility | H2O: soluble0.1 M at 20 °C, clear, colorless to faintly yellow |
| Storage temperature | −20°C |
| Impurities tested | ≤0.1% Insoluble matter |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Cation traces | Al: ≤0.0005%Ca: ≤0.001%Cu: ≤0.0005%Fe: ≤0.001%K: ≤0.005%Mg: ≤0.002%Pb: ≤0.001%Zn: ≤0.0005% |
| Anion traces | sulfate (SO42-): ≤0.05% |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Naphthol AS-BI phosphate disodium salt hydrate used for?
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Naphthol AS-BI phosphate disodium salt hydrate is primarily used as an enzyme substrate in biochemical and histochemical applications for the detection and visualization of enzyme activity, particularly phosphatases.
What is the CAS number and formula for Naphthol AS-BI phosphate disodium salt hydrate?
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The CAS number is 207569-04-8. The empirical formula is C18H13BrNNa2O6P · xH2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Naphthol AS-BI phosphate disodium salt hydrate with an assay of ≥99.5%. We do not represent this product as USP, BP, or EP grade.
How should Naphthol AS-BI phosphate disodium salt hydrate be handled and stored?
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Handle with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Store at −20°C. The substance has a WGK Germany rating of 3, indicating it is hazardous.
How is this chemical shipped or exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals. This product can be shipped and exported internationally, adhering to all relevant regulations.
How can I request a sample or a quote for Naphthol AS-BI phosphate disodium salt hydrate?
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Please contact Tech Serve Solutions directly through our website or sales representatives to request a sample or a quotation for Naphthol AS-BI phosphate disodium salt hydrate.
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