Naphthol AS-BI N-acetyl-β-D-glucosaminide
Naphthol AS-BI N-acetyl-β-D-glucosaminide (CAS: 3395-37-7) is a specialized biochemical reagent with the molecular formula C26H27BrN2O8 and a molecular weight of 575.41 g/mol. This compound serves as a valuable chromogenic substrate, primarily utilised in enzymatic assays and histochemical staining. Its ability to detect specific enzyme activities makes it indispensable in various biological and biochemical research applications, including molecular biology and proteomics.
- IUPAC
- 2-Naphthalenecarboxamide, 3-[[2-(acetylamino)-2-deoxy-β-D-glucopyranosyl]oxy]-7-bromo-N-(2-methoxyphenyl)-,Naphthol AS-BI 2-acetamido-2-deoxy-β-D-glucopyranoside

Enzyme Substrate for Detection
Naphthol AS-BI N-acetyl-β-D-glucosaminide acts as a chromogenic substrate for detecting specific enzyme activities, particularly those involved in glycosaminoglycan metabolism. Upon enzymatic cleavage, it yields a detectable coloured product, facilitating quantitative and qualitative analyses in biochemical research.
Histochemical Staining
This compound is employed in histochemical applications for the insoluble detection of enzyme activity within biological tissues. It is a critical reagent for visualizing and localising specific enzymatic processes in cell biology and pathology studies.
Molecular Biology and Proteomics Research
Utilised in advanced research settings, including molecular biology and proteomics, this substrate aids in the characterization of enzymes and their roles in biological pathways. Its specificity supports detailed investigation into post-translational modifications and enzyme function.
| Molecular weight | 575.41 |
|---|---|
| Empirical formula | C26H27BrN2O8 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Naphthol AS-BI N-acetyl-β-D-glucosaminide used for?
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Naphthol AS-BI N-acetyl-β-D-glucosaminide is primarily used as a chromogenic substrate for the detection of enzyme activity in biochemical assays and histochemical staining. It is valuable in molecular biology, proteomics, and glycobiology research.
What is the CAS number and formula for Naphthol AS-BI N-acetyl-β-D-glucosaminide?
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The CAS number for Naphthol AS-BI N-acetyl-β-D-glucosaminide is 3395-37-7, and its empirical formula is C26H27BrN2O8.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Naphthol AS-BI N-acetyl-β-D-glucosaminide typically as a research or technical grade chemical. We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade unless explicitly stated otherwise in the product specifications. Please refer to the specific Certificate of Analysis for lot-specific purity details.
How should Naphthol AS-BI N-acetyl-β-D-glucosaminide be handled safely?
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Handle Naphthol AS-BI N-acetyl-β-D-glucosaminide with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The compound carries a WGK Germany rating of 3, indicating a high hazard to water.
How is Naphthol AS-BI N-acetyl-β-D-glucosaminide packed, shipped, and exported?
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Tech Serve Solutions packs, ships, and exports chemicals in compliance with all relevant international and national regulations. Packaging is designed to ensure product integrity and safety during transit. We cater to global research and industrial clients.
How can I request a sample or a quote for Naphthol AS-BI N-acetyl-β-D-glucosaminide?
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To request a sample or a quote for Naphthol AS-BI N-acetyl-β-D-glucosaminide, please contact our sales department through the TSS website or by calling our customer service line. Provide the product name and CAS number for prompt assistance.
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