Naphthol AS-D chloroacetate
Naphthol AS-D chloroacetate (CAS 35245-26-2), with empirical formula C20H16ClNO3 and a molecular weight of 353.80, is a valuable biochemical reagent. It serves as a specific substrate for esterase enzymes, facilitating their detection and quantification in various biological and biochemical research applications. Its precise action as an enzyme substrate makes it indispensable for studies involving esterase activity.
- IUPAC
- 3-Hydroxy-2-naphthoic-o-toluidide chloroacetate
- Synonyms
- 3-Hydroxy-2-naphthoic-o-toluidide chloroacetate

Esterase Enzyme Substrate
Naphthol AS-D chloroacetate acts as a chromogenic substrate for the detection and assay of esterase enzyme activity. Upon enzymatic hydrolysis, it releases a naphthol derivative that can be further coupled to form a coloured precipitate, allowing for quantitative or qualitative analysis of enzyme presence and activity.
Biochemical Research
This compound is utilised in biochemical research laboratories for a variety of studies, including enzyme kinetics, purification, and characterisation. Its specific reactivity enables researchers to investigate the role and function of esterases in different biological systems.
Histochemistry
As a substrate for esterases, Naphthol AS-D chloroacetate can be applied in histochemical staining techniques to localise enzyme activity within tissue samples. This aids in the study of cellular and tissue biology and pathology.
| Molecular weight | 353.80 |
|---|---|
| Empirical formula | C20H16ClNO3 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Naphthol AS-D chloroacetate used for?
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Naphthol AS-D chloroacetate is primarily used as a substrate for esterase enzymes in biochemical research, histochemistry, and for enzyme activity assays.
What are the CAS number and formula for Naphthol AS-D chloroacetate?
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The CAS number is 35245-26-2 and the empirical formula is C20H16ClNO3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this compound as a research or technical grade chemical. We do not represent it as USP, BP, EP or pharmacopoeia grade unless explicitly stated in the product specifications. Purity information is available on the Certificate of Analysis.
What are the safety and handling precautions for Naphthol AS-D chloroacetate?
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Handling requires appropriate personal protective equipment, including eyeshields and gloves. A respirator filter of type N95 (US) or P1 (EN143) is recommended. The substance is assigned a WGK Germany classification of 3, indicating a high hazard to water.
How is Naphthol AS-D chloroacetate packed, shipped, and exported?
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Products are typically packed in appropriate containers for stability and safety during transit. Tech Serve Solutions is a global supplier and exporter, adhering to all relevant shipping regulations for chemical substances.
How can I request a sample or quote for Naphthol AS-D chloroacetate?
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To request a sample or a quotation, please contact our sales department through the website or by phone, providing your specific requirements.
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