N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride
N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride (CAS 637-01-4) is a key organic chemical compound with the formula C<sub>6</sub>H<sub>4</sub>[N(CH<sub>3</sub>)<sub>2</sub>]<sub>2</sub>·2HCl and a molecular weight of 237.17 g/mol. Supplied by Tech Serve Solutions with an assay of ≥97.0% (AT), it is primarily utilised as a sensitive reagent in microbiology for the identification of cytochrome oxidase-positive aerobic microorganisms. Its utility extends to analytical chemistry as a derivatization reagent.
- IUPAC
- TMPPD,Wurster’s reagent
- Synonyms
- N′-Tetramethyl-p-phenylenediamine dihydrochloride637-01-4C6H4[N(CH3)2]2·2HClTMPPDWurster’s reagent

Oxidase Test Reagent
N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride is a critical component in the oxidase test, a standard microbiological procedure. A 1% aqueous solution is used to detect the presence of the enzyme cytochrome oxidase in bacteria, aiding in their classification and identification.
Analytical Reagent
This compound serves as a valuable reagent in various analytical chemistry applications. It is employed in derivatization reactions, particularly in chromatography, to enhance the detection and separation of specific analytes.
Chemical Synthesis Building Block
As an organic building block, N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride can be utilised in the synthesis of more complex organic molecules. Its diamine structure offers potential for diverse chemical transformations.
| Molecular weight | 237.17 |
|---|---|
| Linear formula | C6H4[N(CH3)2]2·2HCl |
| Assay | ≥97.0% (AT) |
| Solubility | H2O: soluble500 mg/10 mL |
| Application | Test reagent in microbiology for the classification of cytochrome oxidase positive aerobic microorganisms.; For oxidase reagent, use a 1% aqueous solution of N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride |
| Melting point | 222-224 °C(lit.) |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride used for?
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N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride is primarily used as a test reagent in microbiology for the classification of cytochrome oxidase-positive aerobic microorganisms. It is also used as an analytical reagent and a building block in chemical synthesis.
What is the CAS number and chemical formula for N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride?
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The CAS number is 637-01-4, and the linear chemical formula is C<sub>6</sub>H<sub>4</sub>[N(CH<sub>3</sub>)<sub>2</sub>]<sub>2</sub>·2HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride with an assay of ≥97.0% (AT). TSS does not represent this product as USP, BP, EP or other pharmacopoeial grades unless explicitly stated in the product data.
How should N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride be handled safely?
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This compound is a warning substance that causes skin irritation (H315), serious eye irritation (H319), and may cause respiratory irritation (H335). Always use appropriate personal protective equipment, including a dust mask type N95 (US), eyeshields, and gloves. Handle in a well-ventilated area. Refer to the Safety Data Sheet (SDS) for full details.
Is N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride shipped internationally?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride, and can accommodate international shipping requests.
How can I request a sample or quote for N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride?
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To request a sample or a quote, please contact our sales team through the website or by phone. Provide the product name and CAS number (637-01-4) for efficient processing.
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