N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid
N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid (CAS 36930-63-9) is a fluorescent probe with the linear formula ICH₂CONHCH₂CH₂NHC₁₀H₆SO₃H and a molecular weight of 434.25. This compound is recognised for its utility in biological research, primarily for labelling protein sulfhydryls. Its applications include studying protein conformational changes, fluorescence anisotropy, and protein-protein interactions, offering valuable insights in molecular biology and biochemistry.
- IUPAC
- 1,5-I-AEDANS,5-[2-(Iodoacetamido)ethylamino]naphthalene-1-sulfonic acid,N-Iodoacetyl-N′-(5-sulfo-1-naphthyl)ethylenediamine,Hudson-Weber-Reagent
- Synonyms
- 1,5-I-AEDANS5-[2-(Iodoacetamido)ethylamino]naphthalene-1-sulfonic acidN-Iodoacetyl-N′-(5-sulfo-1-naphthyl)ethylenediamineHudson-Weber-Reagent
Fluorescent Labelling of Proteins
N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid is employed to covalently label sulfhydryl groups on proteins. This allows for the introduction of a fluorescent tag, enabling detection and analysis via fluorescence spectroscopy.
Study of Protein Conformations
By attaching this probe to specific sites on a protein, researchers can monitor conformational shifts using fluorescence anisotropy techniques. Changes in fluorescence intensity and polarization can indicate structural alterations.
Investigation of Protein Interactions
The fluorescent properties of the labelled protein can be utilised to study protein-protein interactions. Changes in fluorescence signals can provide evidence for binding events or complex formation.
Biochemical Assays
This reagent serves as a valuable tool in various biochemical assays, particularly those investigating enzyme activity or cellular processes where protein sulfhydryl modification is relevant.
| Molecular weight | 434.25 |
|---|---|
| Linear formula | ICH2CONHCH2CH2NHC10H6SO3H |
| Assay | ≥95% (HPLC) |
| Storage temperature | 2-8°C |
| Application | N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid (1,5-I-AEDANS) is a fluorescence probe used to label protein sulfhydryls to study conformational changes, fluorescence anisotropy and protein:protein interactions. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 336 nm; λem 490 nm (pH 8.0)λex 337 nm; λem 498 nm (after derivatization with 2-mercaptoethanol) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid primarily used for?
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It is primarily used as a fluorescence probe to label protein sulfhydryls, facilitating studies on protein conformational changes, fluorescence anisotropy, and protein-protein interactions.
What are the CAS number and linear formula for this compound?
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The CAS number is 36930-63-9, and the linear formula is ICH₂CONHCH₂CH₂NHC₁₀H₆SO₃H.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥95% (HPLC). We do not represent it as USP, BP, EP, or any other pharmacopoeial grade.
How should N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid be handled?
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When handling, it is essential to use personal protective equipment including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The compound has a WGK Germany rating of 3, indicating it is hazardous to water.
How is this chemical packed and shipped?
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This chemical is typically stored at 2-8°C. Packaging and shipping procedures adhere to international regulations for chemical transport.
How can I request a sample or a quote for N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid?
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To request a sample or a quote, please contact our sales department through the TSS website or by calling our customer service line.
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