N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone

CAS 115930-63-7C27H25N3O5Biochemicals & Reagents

N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone (CAS 115930-63-7) is a chemical compound with the molecular formula C27H25N3O5 and a molecular weight of 471.50 g/mol. This fluorogenic substrate is specifically designed for the assay of proteases and peptidases. Its principal use lies in the detection and quantification of enzyme activity, particularly for enzymes like α-chymotrypsin and trypsin, by emitting fluorescence upon cleavage.

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N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone (C27H25N3O5) — chemical structure, CAS 115930-63-7; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsChymotrypsinalphaDetectionEnzyme SubstratesEnzymesInhibitorsand SubstratesFluorescent ProbesLabels

01 /Applications

Protease Activity Assay

N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone serves as a sensitive fluorogenic substrate for assaying the activity of various proteases and peptidases. Upon enzymatic cleavage, it releases a fluorescent product, allowing for quantitative measurement of enzyme kinetics.

Chymotrypsin Detection

This compound is particularly well-suited for the detection and quantification of α-chymotrypsin activity. The cleavage of the substrate by α-chymotrypsin leads to a measurable change in fluorescence, enabling precise activity profiling.

Trypsin Activity Measurement

The substrate is also applicable for the assay of trypsin, another key protease. Its use allows researchers to accurately determine trypsin levels and study its enzymatic function in biological or biochemical contexts.

Enzyme Research and Development

Utilised in biochemical research, this fluorogenic substrate aids in the characterisation of enzyme mechanisms, screening of enzyme inhibitors, and development of novel enzymatic assays. Its fluorescence properties are valuable for high-throughput screening applications.

02 /Properties

Molecular weight	471.50
Empirical formula	C27H25N3O5
Assay	≥98.0% (HPLC)
Grade	for fluorescence
Solubility	DMF: solubleDMSO: soluble
Storage temperature	2-8°C
Application	N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone is a fluorogenic substrate used to assay proteases/peptidases such as α-chymotrypsin and trypsin.
Melting point	272 °C(lit.)

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

115930-63-7

MDL number

MFCD01863061

05 /Additional specifications

Fluorescence	λex 398 nm; λem 440 nm in 0.1 M Tris pH 8.0 (fluoroescence decreases upon cleavage with α-chymotrypsin)

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone used for?

N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone is a fluorogenic substrate used to assay proteases/peptidases such as α-chymotrypsin and trypsin.

What is the CAS number and chemical formula for N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone?

The CAS number is 115930-63-7, and the empirical formula (Hill notation) is C27H25N3O5.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone with an assay of ≥98.0% (HPLC) and a grade 'for fluorescence'. TSS does not represent this product as USP, BP, EP, or any other pharmacopoeia grade.

What are the safety and handling precautions for this chemical?

When handling N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone, it is essential to use safety measures including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The compound has a WGK Germany classification of 3.

How is this product shipped and exported?

This product requires storage at 2-8°C. Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, capable of handling international shipments.

How can I request a quote or sample?

To request a quote or sample of N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone, please contact our sales team through the website or designated contact channels.