N-(4-Aminobutyl)-N-ethylisoluminol
N-(4-Aminobutyl)-N-ethylisoluminol (CAS 66612-29-1) is a chemiluminescent compound with the molecular formula C14H20N4O2. It is a derivative of isoluminol, widely recognised for its applications in highly sensitive detection methods. This compound is primarily utilised as a substrate in various biochemical assays, particularly those involving peroxidases, for generating light-based signals.
- IUPAC
- 4-(N1-Ethyl-4-aminobutylamino)phthalic hydrazide,6-[N-(4-Aminobutyl)-N-ethylamino]-2,3-dihydro-1,4-phthalazinedione,ABEI
- Synonyms
- 4-(N1-Ethyl-4-aminobutylamino)phthalic hydrazide6-[N-(4-Aminobutyl)-N-ethylamino]-2,3-dihydro-1,4-phthalazinedioneABEI

Chemiluminescent Assays
This compound serves as a key substrate in chemiluminescent immunoassays (CLIAs) and other detection systems. It enables the sensitive quantification of analytes by producing light upon enzymatic reaction, often with peroxidases.
Enzyme Substrate
As a specialised substrate, it is employed in biochemical research and diagnostics to detect the activity of enzymes such as horseradish peroxidase (HRP). The resulting light emission can be measured to determine enzyme concentration or activity.
Biochemical Research
N-(4-Aminobutyl)-N-ethylisoluminol is utilised in fundamental biochemical research for developing and optimising detection methodologies. Its luminescent properties make it valuable for studying biological processes at low concentrations.
| Molecular weight | 276.33 |
|---|---|
| Empirical formula | C14H20N4O2 |
| Assay | ≥90% |
| Storage temperature | 2-8°C |
| Melting point | 259-260 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N-(4-Aminobutyl)-N-ethylisoluminol used for?
+
N-(4-Aminobutyl)-N-ethylisoluminol is primarily used as a chemiluminescent substrate in biochemical assays, particularly for detecting peroxidase activity, enabling sensitive light-based detection methods in research and diagnostics.
What is the CAS number and molecular formula for N-(4-Aminobutyl)-N-ethylisoluminol?
+
The CAS number for N-(4-Aminobutyl)-N-ethylisoluminol is 66612-29-1, and its molecular formula is C14H20N4O2.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies N-(4-Aminobutyl)-N-ethylisoluminol with an assay of ≥90%. This product is intended for research use and is not represented as USP, BP, EP, or pharmacopoeia grade.
How should N-(4-Aminobutyl)-N-ethylisoluminol be handled and stored?
+
Handle N-(4-Aminobutyl)-N-ethylisoluminol with appropriate personal protective equipment, including eyeshields, gloves, and a P1/N95 respirator. Store at 2-8°C.
Is N-(4-Aminobutyl)-N-ethylisoluminol a controlled substance?
+
The provided data does not indicate that N-(4-Aminobutyl)-N-ethylisoluminol is a controlled substance.
How can I request a sample or quote for N-(4-Aminobutyl)-N-ethylisoluminol?
+
To request a sample or a quotation for N-(4-Aminobutyl)-N-ethylisoluminol, please contact our sales department via the website or directly by phone.
How is N-(4-Aminobutyl)-N-ethylisoluminol shipped?
+
N-(4-Aminobutyl)-N-ethylisoluminol is typically shipped under controlled temperature conditions to maintain its integrity. As a global supplier, Tech Serve Solutions complies with all export regulations.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents

(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more

3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis

3-Methyl-1,5-pentanediol
Chemical Synthesis

3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis