N-(5-Fluoresceinyl)maleimide

CAS 75350-46-8C24H13NO7Biochemicals & Reagents

N-(5-Fluoresceinyl)maleimide (CAS: 75350-46-8; Formula: C24H13NO7; MW: 427.36) is a highly fluorescent labelling reagent. It is specifically designed for covalent conjugation to molecules containing thiol groups. This compound is invaluable in biochemical and biological research for modifying proteins and peptides, enabling sensitive detection and analysis through fluorescence microscopy and spectroscopy.

IUPAC: 5-Maleimido-fluorescein
Synonyms: 5-Maleimido-fluorescein

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N-(5-Fluoresceinyl)maleimide (C24H13NO7) — chemical structure, CAS 75350-46-8; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsWavelength Index

01 /Applications

Fluorescence Labelling of Proteins

N-(5-Fluoresceinyl)maleimide reacts selectively with cysteine thiol groups on proteins. This allows for the introduction of a fluorescent tag, facilitating downstream detection and quantification in various biological assays.

Peptide Modification

This maleimide derivative can be used to label peptides that possess accessible sulfhydryl groups. The resulting fluorescently labelled peptides are useful for studying peptide-protein interactions or for tracking peptide localisation.

Bioconjugation Reagent

The compound serves as a versatile reagent for bioconjugation, enabling the covalent attachment of the fluorescein moiety to biomolecules. This is crucial for developing fluorescent probes for diagnostic and research purposes.

02 /Properties

Molecular weight	427.36
Empirical formula	C24H13NO7
Assay	≥90% (HPLC)
Application	N-(5-Fluoresceinyl)maleimide (5-FM) is used to fluorescence label molecules such as proteins and peptides via their thiol groups.
Suitability	corresponds for coupling to thiolssuitable for fluorescence

03 /Safety & handling

GHS hazard pictogram: Harmful / irritant (GHS07) — Harmful / irritant

Warning

Hazard statements

H302Harmful if swallowed

Protective equipment	dust mask type N95 (US), Eyeshields, Gloves
Water hazard class (WGK, DE)	3
Hazard codes (EU)	Xn
Risk statements (R)	22

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

75350-46-8

05 /Additional specifications

Fluorescence	λex 492 nm; λem 518 nm in 0.1 M Tris pH 9.0, 2-mercaptoethanol

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is N-(5-Fluoresceinyl)maleimide used for?

N-(5-Fluoresceinyl)maleimide is used to fluorescence label molecules such as proteins and peptides via their thiol groups, enabling sensitive detection and analysis.

What is the CAS number and formula for N-(5-Fluoresceinyl)maleimide?

The CAS number is 75350-46-8 and the empirical formula is C24H13NO7.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies N-(5-Fluoresceinyl)maleimide with an assay of ≥90% (HPLC). This is suitable for research purposes and coupling to thiols. We do not represent this product as USP, BP, or EP grade.

What are the safety precautions when handling N-(5-Fluoresceinyl)maleimide?

N-(5-Fluoresceinyl)maleimide is classified as a GHS07 'Warning' substance with hazard statement H302 (Harmful if swallowed). Appropriate personal protective equipment, including a dust mask (type N95 US), eyeshields, and gloves, should be used. Handle with care to avoid ingestion and dust formation.

Is this product available for export?

Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including N-(5-Fluoresceinyl)maleimide, and can assist with export arrangements.

How can I request a sample or quote?

To request a sample or a quote for N-(5-Fluoresceinyl)maleimide, please contact our sales team through the website or by phone, providing your specific requirements and delivery details.