N-(4-Methylumbelliferyl)maleimide
N-(4-Methylumbelliferyl)maleimide (CAS 211565-47-8), with the molecular formula C14H9NO4, is a biochemical reagent used for labeling. It is particularly suitable for conjugation with thiols due to its maleimide group. This compound exhibits fluorescence with excitation and emission maxima at 330 nm and 393 nm, respectively, making it valuable in various biochemical and biological research applications for detection and imaging.
- IUPAC
- 7-Maleimido-4-methylcoumarin
Thiol Labeling Reagent
N-(4-Methylumbelliferyl)maleimide is designed for the selective labeling of sulfhydryl (thiol) groups in biomolecules such as proteins and peptides. The maleimide functionality reacts efficiently with free thiols under mild conditions.
Fluorescent Probe Development
Its intrinsic fluorescence properties, with excitation at 330 nm and emission at 393 nm, allow N-(4-Methylumbelliferyl)maleimide to serve as a fluorescent tag. This enables sensitive detection and quantification of molecules to which it is conjugated.
Bioconjugation Chemistry
This reagent is employed in bioconjugation strategies to attach the fluorescent 4-methylumbelliferyl moiety to biomolecules. This is useful for tracking, imaging, or studying molecular interactions in biological systems.
| Molecular weight | 255.23 |
|---|---|
| Empirical formula | C14H9NO4 |
| Storage temperature | 2-8°C |
| Suitability | corresponds for coupling to thiols |
| Fluorescence | λex 330 nm; λem 393 nm in 0.1 M phosphate pH 7.0, 2-mercaptoethanol |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N-(4-Methylumbelliferyl)maleimide used for?
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N-(4-Methylumbelliferyl)maleimide is primarily used as a fluorescent labeling reagent for thiols (sulfhydryl groups) in biochemical applications. It allows for the detection and quantification of molecules containing free thiols through fluorescence.
What is the CAS number and formula for N-(4-Methylumbelliferyl)maleimide?
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The CAS number is 211565-47-8, and the empirical formula is C14H9NO4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies N-(4-Methylumbelliferyl)maleimide as a research grade chemical. The specific purity is detailed in the product's Certificate of Analysis, and TSS does not represent this product as USP, BP, EP, or any pharmacopoeia grade.
What are the safety and handling recommendations for N-(4-Methylumbelliferyl)maleimide?
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According to the GHS classification, this compound has a WGK Germany rating of 3, indicating it is hazardous for water. Handle with appropriate personal protective equipment in a well-ventilated area. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal information.
How is N-(4-Methylumbelliferyl)maleimide typically stored and shipped?
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N-(4-Methylumbelliferyl)maleimide should be stored at 2-8°C. TSS ensures that all products are packed and shipped in compliance with international regulations for chemical export.
How can I request a quote or sample for N-(4-Methylumbelliferyl)maleimide?
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To request a quote or a sample of N-(4-Methylumbelliferyl)maleimide, please contact our sales department via the website or provide your details through the 'Enquire Now' form.
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