MES monohydrate
MES monohydrate (CAS: 145224-94-8), with the empirical formula C6H13NO4S · H2O and a molecular weight of 213.25 g/mol, is a zwitterionic N-heterocyclic compound widely recognised as a biological buffer. It offers a useful buffering range of pH 5.5-6.7, making it suitable for various biochemical and biological research applications where maintaining a stable pH environment is critical for experimental integrity.
- IUPAC
- 2-(N-Morpholino)ethanesulfonic acid,4-Morpholineethanesulfonic acid monohydrate
Biological Buffering
MES monohydrate is primarily employed as a biological buffer in a pH range of 5.5 to 6.7. Its zwitterionic nature and low UV absorbance make it suitable for cell culture media, electrophoresis, and enzyme assays.
Biochemical Research
It serves as a crucial reagent in biochemical research for maintaining stable pH conditions during enzymatic reactions, protein purification, and other sensitive biological experiments.
Electrophoresis Applications
The buffer's properties are advantageous in electrophoresis techniques, providing stable ionic strength and pH for effective separation of biomolecules.
| Molecular weight | 213.25 |
|---|---|
| Empirical formula | C6H13NO4S · H2O |
| Assay | ≥99.5% (T) |
| Solubility | H2O: soluble0.5 M at 20 °C, clear, colorless |
| pH | 2.5-4.0 (25 °C, 0.5 M in H2O) |
| Impurities tested | DNases, none detectedInsoluble matter, passes filter testPhosphatases, none detectedProteases, none detectedRNases, none detected |
| Melting point | >300 °C(lit.) |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
| Anion traces | chloride (Cl-): ≤50 mg/kgsulfate (SO42-): ≤50 mg/kg |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Uv Absorption | λ: 260 nm Amax: 0.025λ: 280 nm Amax: 0.020 |
|---|---|
| Useful Ph Range | 5.5 - 6.7 |
| Ign Residue 900 °C | ≤0.05% (as SO4) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is MES monohydrate used for?
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MES monohydrate is primarily used as a biological buffer in the pH range of 5.5-6.7 for biochemical and biological research applications, including cell culture and electrophoresis.
What are the CAS number and formula for MES monohydrate?
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The CAS number for MES monohydrate is 145224-94-8, and its empirical formula is C6H13NO4S · H2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies MES monohydrate with an assay of ≥99.5% (T). This product is supplied for research and laboratory use and is not represented as USP, EP, or any other pharmacopoeia grade.
What are the main hazards associated with MES monohydrate?
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MES monohydrate is a warning substance that may cause skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335). Appropriate personal protective equipment including a dust mask (type N95), eyeshields, and gloves should be worn.
How is MES monohydrate packed and shipped?
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Tech Serve Solutions exports MES monohydrate globally. Specific packaging details depend on the quantity ordered and destination requirements, adhering to international shipping regulations.
How can I request a sample or quote for MES monohydrate?
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To request a sample or a quote for MES monohydrate, please contact our sales team via the website or by phone, providing your specific requirements.
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