Fluo 3-AM

CAS 121714-22-5C51H50Cl2N2O23Biochemicals & Reagents

Fluo 3-AM (CAS: 121714-22-5), a cell-permeable fluorescent indicator, is crucial for measuring intracellular calcium (Ca 2+ ) concentrations. This non-fluorescent compound becomes fluorescent upon intracellular hydrolysis and/or binding with Ca 2+ . With a lower binding affinity compared to Fura 2, Fluo 3-AM enables the measurement of higher peaks in calcium transients, making it invaluable in cell biology research for tracking dynamic changes in cellular calcium levels.

IUPAC: 4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4′-methyl-2,2′(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester

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Fluo 3-AM (C51H50Cl2N2O23) — chemical structure, CAS 121714-22-5; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsCalcium IndicatorsChelators and Ion ProbesFluorescent ProbesLabelsParticles and Stains

01 /Applications

Intracellular Calcium Imaging

Fluo 3-AM serves as a cell-permeable indicator for quantifying intracellular calcium (Ca 2+ ) dynamics. Its fluorescence is activated by intracellular hydrolysis and calcium binding, allowing for visualisation and measurement of calcium transients in living cells.

Research in Cell Signalling

The compound's capacity to measure higher peaks of Ca 2+ transients, due to its lower binding affinity, makes it suitable for studying rapid cellular signalling events involving calcium flux. It aids researchers in understanding cell physiology and disease mechanisms.

Biochemical Assays

Fluo 3-AM is employed in various biochemical assays where monitoring intracellular calcium is essential. Its application extends to research areas investigating ion channel activity, receptor function, and cellular responses to stimuli.

02 /Properties

Empirical formula	C51H50Cl2N2O23
Assay	~70%
Storage temperature	−20°C
Application	Cell permeable fluorescent indicator of intracellular Ca2+; non-fluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2+; lower binding affinity allows measurement of higher peaks of Ca2+ transients than with Fura 2.

03 /Identifiers & registry

CAS number

121714-22-5

04 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

05 /Frequently asked questions

What is Fluo 3-AM used for?

Fluo 3-AM is used as a cell-permeable fluorescent indicator for measuring intracellular calcium (Ca 2+ ) concentrations, particularly for detecting higher peaks of calcium transients in biological research.

What is the CAS number and formula for Fluo 3-AM?

The CAS number for Fluo 3-AM is 121714-22-5, and its empirical formula is C51H50Cl2N2O23.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Fluo 3-AM with an assay of approximately 70%. It is supplied as a research-grade chemical and is not represented as USP, BP, EP, or pharmaceutical grade.

What are the safety considerations for handling Fluo 3-AM?

Fluo 3-AM has a WGK Germany classification of 3, indicating it is hazardous to water. Handle with appropriate personal protective equipment in a well-ventilated area and avoid release into the environment.

How is Fluo 3-AM typically packed and shipped?

Fluo 3-AM is stored at -20°C. Tech Serve Solutions exports fine chemicals globally, ensuring appropriate packaging and documentation for safe transit.