Fluo-3
Fluo-3 (CAS: 123632-39-3; Formula: C36H30Cl2N2O13) is a highly sensitive fluorescent indicator designed for the detection of intracellular calcium ions (Ca2+). With a molecular weight of 769.54 g/mol, this compound exhibits a significant increase in fluorescence intensity (approximately 40-fold) upon binding with calcium. It is particularly valuable for quantitative measurements in flow cytometry and confocal laser scanning microscopy, compatible with standard 488 nm excitation sources.
- IUPAC
- 4-(2,7-Dichloro-6-hydroxy-3-oxo-9-xanthenyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid
Intracellular Calcium Imaging
Fluo-3 serves as a critical fluorescence indicator for monitoring intracellular calcium (Ca2+) levels. Its fluorescence intensity dramatically increases upon calcium binding, allowing for precise quantitative analysis.
Flow Cytometry
This reagent is suitable for use in flow cytometry applications, enabling researchers to quantify intracellular calcium dynamics in cell populations with visible light excitation, typically from argon laser sources.
Confocal Laser Scanning Microscopy
Fluo-3 is employed in confocal laser scanning microscopy for high-resolution imaging of calcium transients within cells, utilizing its favourable spectral properties and excitation compatibility with 488 nm lasers.
| Molecular weight | 769.54 |
|---|---|
| Empirical formula | C36H30Cl2N2O13 |
| Assay | ~70% |
| Solubility | H2O: soluble |
| Storage temperature | −20°C |
| Application | Fluo-3 and related molecule Fluo3/AM are used as a fluorescence indicator of intracellular calcium (Ca2+). Fluo-3 may be use for flow cytometry and confocal laser scanning microscopy using visible light excitation (compatible with argon laser sources operating at 488 nm). Fluorescence intensity increases about 40-fold after calcium binding. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 506 nm; λem 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca2+ |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Fluo-3 used for?
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Fluo-3 is used as a fluorescence indicator for intracellular calcium (Ca2+), suitable for flow cytometry and confocal microscopy, with fluorescence intensity increasing significantly upon calcium binding.
What are the CAS number and formula for Fluo-3?
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The CAS number for Fluo-3 is 123632-39-3 and its empirical formula is C36H30Cl2N2O13.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Fluo-3 with an assay of approximately 70%. This product is supplied for research use and is not represented as USP, BP, EP, or pharmacopoeia grade.
What are the safety precautions for handling Fluo-3?
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Handling Fluo-3 requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used. The compound is classified with WGK Germany 3.
How is Fluo-3 typically stored and shipped?
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Fluo-3 is stored at -20°C. Tech Serve Solutions can pack, ship, and export this chemical globally.
How can I request a sample or quote for Fluo-3?
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To request a sample or quote for Fluo-3, please contact our sales team through the website or by phone.
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