Fluorescein
Fluorescein (CAS: 2321-07-5, C20H12O5, MW: 332.31) is a vibrant organic compound widely recognised for its intense fluorescence. This dye exhibits strong absorption in the visible spectrum and emits light upon excitation, making it invaluable in various analytical and biological applications. Available from Tech Serve Solutions in a 'for fluorescence' grade, it serves critical roles in microscopy, diagnostics, and as a sensitive indicator.
- IUPAC
- Acid Yellow 73
Fluorescent Dye and Probe
Fluorescein's strong fluorescence properties make it a key component in fluorescent probes and labels. It is used extensively in biological research to stain cells and tissues, enabling visualisation of cellular structures and processes under fluorescent microscopy.
Adsorption Indicator
As an adsorption indicator, Fluorescein is employed in analytical chemistry for titrations. Its colour change at specific pH levels or ion concentrations allows for precise endpoint determination in various quantitative analyses.
Analytical Reagent
This compound serves as a versatile analytical reagent. Its well-defined spectral properties (λmax 496 nm) and fluorescence characteristics (λex 490 nm; λem 514 nm) are utilised in spectrophotometric and fluorometric assays.
Research and Development
In R&D settings, Fluorescein is instrumental for developing new diagnostic tools and imaging agents. Its compatibility with various biological systems and its distinct optical properties support innovation in life sciences.
| Molecular weight | 332.31 |
|---|---|
| Empirical formula | C20H12O5 |
| Grade | for fluorescence |
| Suitability | passes test for TLCpasses test for UV spectrum |
| Melting point | 320 °C(lit.) |
Hazard statements
- H319Causes serious eye irritation
Precautionary statements
- P305IF IN EYES
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36 |
| Safety statements (S) | 26 |
| Ignition residue | ≤0.25% (as SO4) |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 45350 |
|---|---|
| Gene Information | human ... PTPN1(5770), PTPRF(5792) |
| Absorption | λmax 496 nm |
| Fluorescence | λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Fluorescein primarily used for?
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Fluorescein is primarily used as a fluorescent dye and probe in biological research, as an adsorption indicator in analytical chemistry, and as a general analytical reagent due to its distinct optical properties.
What are the CAS number and formula for Fluorescein?
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The CAS number for Fluorescein is 2321-07-5 and its molecular formula is C20H12O5.
What grade and purity does Tech Serve Solutions supply for Fluorescein?
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Tech Serve Solutions supplies Fluorescein in a 'for fluorescence' grade, suitable for its intended applications. This grade is not represented as USP, BP, or EP.
What are the safety considerations when handling Fluorescein?
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Fluorescein is classified with a GHS07 symbol and a 'Warning' signal word, carrying the hazard statement H319 for serious eye irritation. When handling, it is recommended to wear eyeshields, gloves, and a P1 or N95 respirator filter. Always consult the Safety Data Sheet (SDS) for full details.
Is Fluorescein available for export?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including Fluorescein, and can fulfil international orders.
How can I request a quote or sample for Fluorescein?
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To request a quote or sample for Fluorescein, please contact our sales team through the website or by phone, providing the product name and CAS number.
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