Bromocresol Green
Bromocresol Green (CAS 76-60-8), with the empirical formula C21H14Br4O5S and a molecular weight of 698.01 g/mol, is a pH-sensitive triphenylmethane dye. It is widely employed in various colorimetric detection methods. Its applications include use as a tracking dye in DNA agarose gel electrophoresis, for protein determinations, and in charge-transfer complexation processes. In thin-layer chromatography (TLC), it aids in visualising compounds with a pKa below 5.0.
pH Indicator
Bromocresol Green serves as a reliable pH indicator, exhibiting a colour change from yellow to blue within the pH range of 3.8 to 5.4. This property makes it valuable for titrations and general pH monitoring.
Electrophoresis Tracking Dye
Its distinct colour allows Bromocresol Green to function as a tracking dye in DNA agarose gel electrophoresis. It helps visualise the progress of DNA migration through the gel matrix.
Protein Determination
This dye is utilised in methods for determining protein concentrations. It plays a role in specific assays designed for the colorimetric quantification of proteins.
Thin-Layer Chromatography (TLC) Visualisation
In TLC, Bromocresol Green is applied to visualise compounds possessing functional groups with a pKa value lower than 5.0, facilitating their detection on the chromatogram.
| Molecular weight | 698.01 |
|---|---|
| Empirical formula | C21H14Br4O5S |
| Assay | ≥95% |
| Application | Bromocresol Green (BCG) is a pH sensitive triphenylmethane dye useful in a variety of colorimetric detection technologies. BCG is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge-transfer complexation processes. In TLC BCG is used for visualisation of the compounds with functional groups whose pKa is below 5.0. |
| Melting point | 225 °C (dec.)(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Absorption | λmax 423 nm |
|---|---|
| Ph Range | 3.8 - 5.4, yellow to blue |
| Synonym | 3.8 - 5.4, yellow to blue (passes test) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Bromocresol Green used for?
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Bromocresol Green (CAS 76-60-8) is used as a pH indicator (3.8-5.4, yellow to blue), a tracking dye in DNA agarose gel electrophoresis, in protein determinations, in charge-transfer complexation processes, and for visualising compounds in TLC with a pKa below 5.0.
What is the CAS number and empirical formula for Bromocresol Green?
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The CAS number for Bromocresol Green is 76-60-8, and its empirical formula is C21H14Br4O5S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Bromocresol Green with an assay of ≥95%. This product is intended for laboratory and research use; it is not supplied as USP, BP, EP, or pharmacopoeia grade.
How should Bromocresol Green be handled safely?
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Handling Bromocresol Green requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended for respiratory protection. Consult the Safety Data Sheet for full handling instructions.
How is Bromocresol Green packed and shipped?
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Bromocresol Green is carefully packed to ensure stability during transit and is exported globally by Tech Serve Solutions. Specific packaging details can be provided upon request.
How can I request a quote or sample?
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To request a quote or a sample of Bromocresol Green, please contact our sales team through the website or by phone, providing your specific requirements.
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