Bromophenol Blue

CAS 115-39-9C19H10Br4O5SCell Biology

Bromophenol Blue (CAS: 115-39-9) is a brominated analogue of phenolsulfonphthalein and an organic compound with the formula C19H10Br4O5S. Exhibiting a molecular weight of 669.96 g/mol, this substance is widely recognised for its utility as a pH indicator and as a vital tracking dye in various biotechnological applications, particularly in gel electrophoresis.

IUPAC: 3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein,Bromophenol Blue (BPB),Bromphenol Blue Sultone Form
Synonyms: 3′,3′′,5′,5′′-TetrabromophenolsulfonephthaleinBromophenol Blue (BPB)Bromphenol Blue Sultone Form

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Bromophenol Blue (C19H10Br4O5S) — chemical structure, CAS 115-39-9; Life Science, fine chemical supplied by Tech Serve Solutions

Analytical ReagentsAnalytical/ChromatographyCell BiologyHematology and HistologyIndicatorsStains & DyesA to ZStains and DyesTitration

01 /Applications

Tracking Dye in Gel Electrophoresis

Bromophenol Blue serves as an essential tracking dye in both DNA and RNA agarose gel electrophoresis, as well as in protein polyacrylamide gel electrophoresis. It migrates at a rate comparable to 300-500 base pairs of DNA in agarose gels and at the buffer front in protein gels, allowing for visual monitoring of electrophoresis progress.

pH Indicator

This compound functions as a pH indicator, exhibiting distinct colour changes across a specific pH range. Its utility in titrations and general pH determination makes it a valuable reagent in analytical chemistry laboratories.

Histology and Cell Biology Staining

Bromophenol Blue is also employed in certain histological and cell biology applications as a stain or dye. Its properties are beneficial for visualising cellular structures or processes in microscopic examination.

02 /Properties

Molecular weight	669.96
Empirical formula	C19H10Br4O5S
Grade	ACS reagent
Form	powder
Application	Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

115-39-9

MDL number

MFCD00005875

PubChem substance

24847169

Beilstein registry

61698

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is Bromophenol Blue primarily used for?

Bromophenol Blue is primarily used as a tracking dye in gel electrophoresis for DNA, RNA, and protein analysis, and as a pH indicator in chemical analyses.

What are the CAS number and chemical formula for Bromophenol Blue?

The CAS number for Bromophenol Blue is 115-39-9, and its empirical formula is C19H10Br4O5S.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Bromophenol Blue in ACS reagent grade.

What are the safety and handling considerations for Bromophenol Blue?

Handling Bromophenol Blue requires appropriate personal protective equipment, including eyeshields and gloves, and respiratory protection such as a type N95 or P1 respirator filter. It is classified with WGK Germany 2, indicating it is hazardous to water.