Bromocresol Green sodium salt
Bromocresol Green sodium salt (CAS 62625-32-5), with the empirical formula C21H13Br4NaO5S and a molecular weight of 720.00, is a triphenylmethane dye exhibiting pH sensitivity. It functions as a visual indicator in various analytical applications. Key uses include tracking dye in DNA agarose gel electrophoresis, protein determinations, and charge-transfer complexation. It is also employed in Thin Layer Chromatography (TLC) for visualising compounds with specific pKa values.
- IUPAC
- 3′,3″,5′,5″-Tetrabromo-m-cresolsulfonphthalein sodium salt
- Synonyms
- 3′,3″,5′,5″-Tetrabromo-m-cresolsulfonphthalein sodium salt
pH Indicator
As a triphenylmethane dye, Bromocresol Green sodium salt undergoes a distinct colour change with variations in pH, making it a valuable indicator in colorimetric analyses. Its transition range is useful for monitoring specific pH levels in solutions.
Electrophoresis Tracking Dye
This compound serves as an effective tracking dye in DNA agarose gel electrophoresis. Its visual presence helps researchers monitor the progress of DNA migration through the gel matrix during the separation process.
Protein Determination
Bromocresol Green sodium salt can be utilised in methods for determining protein concentrations. It participates in complexation processes that allow for the spectrophotometric quantification of proteins.
Thin Layer Chromatography (TLC)
In TLC applications, Bromocresol Green sodium salt is used for the visualisation of compounds. It is particularly effective for detecting substances with functional groups having a pKa value below 5.0.
| Molecular weight | 720.00 |
|---|---|
| Empirical formula | C21H13Br4NaO5S |
| Density | 0.981 g/mL at 25 °C |
| Solubility | H2O: soluble1 mg/mL |
| Concentration | 0.04 wt. % in H2O |
| Application | Bromocresol Green (BCG) is a pH sensitive triphenylmethane dye useful in a variety of colorimetric detection technologies. BCG is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge-transfer complexation processes. In TLC BCG is used for visualisation of the compounds with functional groups whose pKa is below 5.0. |
| Melting point | 230 °C (dec.)(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Absorption | λmax 616 nm in H2O |
|---|---|
| Synonym | 3.8 - 5.4, yellow to blue |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Bromocresol Green sodium salt used for?
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Bromocresol Green sodium salt is primarily used as a pH indicator, a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and for visualising compounds in Thin Layer Chromatography (TLC).
What are the CAS number and formula for Bromocresol Green sodium salt?
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The CAS number for Bromocresol Green sodium salt is 62625-32-5, and its empirical formula is C21H13Br4NaO5S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Bromocresol Green sodium salt typically as a reagent grade for laboratory and research use. Specific purity and assay details are available on the Certificate of Analysis; TSS does not represent this product as USP, BP, or EP grade.
How should Bromocresol Green sodium salt be handled safely?
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Handling requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Avoid contact with skin and eyes, and ensure adequate ventilation. Refer to the Safety Data Sheet for comprehensive guidance.
How is Bromocresol Green sodium salt packed and shipped?
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Bromocresol Green sodium salt is carefully packed in compliance with international shipping regulations for chemical export. Standard packaging options are available, and specific requirements can be accommodated upon request.
How can I request a quote or sample for Bromocresol Green sodium salt?
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To request a quote or a sample of Bromocresol Green sodium salt, please contact the Tech Serve Solutions sales team via our website or by phone, providing your specific requirements and shipping details.
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