Bromophenol Blue sodium salt
Bromophenol Blue sodium salt (CAS 34725-61-6), chemical formula C19H9Br4NaO5S, is a vibrant blue pH indicator. With a molecular weight of 691.94 g/mol, it is soluble in water. It is primarily utilised as a tracking dye in electrophoresis for nucleic acids and proteins, aiding visualisation during gel separation. Its distinct colour change across a specific pH range also makes it useful in various analytical applications.
- IUPAC
- 3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
- Synonyms
- 3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
Electrophoresis Tracking Dye
Bromophenol Blue sodium salt serves as an effective tracking dye in both agarose and polyacrylamide gel electrophoresis. It allows researchers to visually monitor the progress of nucleic acid or protein separation during the electrophoretic run.
pH Indicator
This compound functions as a pH indicator, exhibiting a colour change from yellow to blue over the pH range of 3.0 to 4.6. This property makes it valuable for titrations and general pH monitoring in laboratory settings.
Biological Staining
Utilised in cell biology and histology, Bromophenol Blue sodium salt can be employed as a stain. It is particularly noted for its application in detecting protein levels, with a detection limit of 10-80 μg.
| Molecular weight | 691.94 |
|---|---|
| Empirical formula | C19H9Br4NaO5S |
| Colour | blue, at pH above 4.6 |
| Density | 0.985 g/mL at 25 °C |
| Concentration | 0.04 wt. % in H2O |
| Application | A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels. |
| Composition | Dye content, 90% |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Absorption | λmax 383 nmλmax 589 nm |
|---|---|
| Ph Range | 3.0 - 4.6, yellow to blue |
| Measuring Range | 10-80 μg protein detection level |
| Synonym | 3.0 - 4.6, yellow to blue |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Bromophenol Blue sodium salt used for?
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Bromophenol Blue sodium salt is primarily used as a tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels. It also functions as a pH indicator with a colour change from yellow to blue over the pH range of 3.0 to 4.6, and is used for protein detection.
What is the CAS number and chemical formula for Bromophenol Blue sodium salt?
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The CAS number is 34725-61-6 and the chemical formula is C19H9Br4NaO5S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Bromophenol Blue sodium salt with a dye content of 90%. We do not represent this product as USP, BP, EP, or any pharmacopoeia grade.
How should Bromophenol Blue sodium salt be handled?
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Handle Bromophenol Blue sodium salt with appropriate personal protective equipment, including eyeshields and gloves. In areas with potential dust exposure, a type N95 (US) or type P1 (EN143) respirator filter is recommended. Consult the Safety Data Sheet for detailed handling information.
Is Bromophenol Blue sodium salt a controlled substance?
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Based on the provided data, Bromophenol Blue sodium salt is not classified as a controlled substance. Its WGK Germany classification is 3.
How is Bromophenol Blue sodium salt packed, shipped, and exported?
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Bromophenol Blue sodium salt is carefully packed and prepared for shipment and export, adhering to all relevant regulations for chemical transport. Please contact TSS for specific shipping details.
How can I request a sample or quote for Bromophenol Blue sodium salt?
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To request a sample or a quote for Bromophenol Blue sodium salt, please contact our sales team via the website or phone. We will be pleased to assist you with your specific requirements.
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