Bis(p-nitrophenyl) phosphate sodium salt
Bis(p-nitrophenyl) phosphate sodium salt (CAS: 4043-96-3; C12H8O8N2PNa; MW: 362.16) is a biochemical reagent primarily used as an enzyme substrate. It is a chromogenic compound that releases p-nitrophenol, which can be quantified spectrophotometrically, upon enzymatic hydrolysis. This property makes it valuable in biochemical assays and enzymatic studies for detecting and quantifying enzyme activity.

Enzyme Substrate
Utilised as a chromogenic substrate for enzymes that hydrolyse phosphate esters, such as alkaline phosphatase. The enzymatic cleavage releases p-nitrophenol, which can be measured to determine enzyme activity.
Biochemical Assays
A key reagent in various biochemical assays and research, particularly for the detection and quantification of specific enzyme levels in biological samples or purified enzyme preparations.
Chromogenic Reagent
Functions as a chromogenic indicator in assays where the release of p-nitrophenol results in a detectable colour change, facilitating quantitative analysis.
| Molecular weight | 362.16 |
|---|---|
| Linear formula | C12H8O8N2PNa |
| Assay | ≥99% (TLC) |
| Storage temperature | −20°C |
| Impurities tested | ≤0.05% free p-nitrophenol |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Bis(p-nitrophenyl) phosphate sodium salt used for?
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Bis(p-nitrophenyl) phosphate sodium salt is primarily used as a chromogenic enzyme substrate in biochemical assays for detecting and quantifying enzyme activity, particularly of phosphatases.
What are the CAS number and formula for Bis(p-nitrophenyl) phosphate sodium salt?
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The CAS number is 4043-96-3 and the molecular formula is C12H8O8N2PNa.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Bis(p-nitrophenyl) phosphate sodium salt with an assay of ≥99% (TLC), containing ≤0.05% free p-nitrophenol. It is supplied as a technical/research grade and is not represented as USP, BP, or EP.
How should Bis(p-nitrophenyl) phosphate sodium salt be handled and stored?
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Handling requires appropriate personal protective equipment, including eyeshields and gloves, and a P1 filter respirator. It should be stored at −20°C. Refer to the Safety Data Sheet for detailed handling instructions.
How is Bis(p-nitrophenyl) phosphate sodium salt packed, shipped, and exported?
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This chemical is carefully packed to ensure stability during transit and is available for global export by Tech Serve Solutions, adhering to all relevant shipping regulations.
How can I request a quote or sample?
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Please contact the Tech Serve Solutions sales team via the website or direct email to request a quote or a sample of Bis(p-nitrophenyl) phosphate sodium salt.
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