BIS-TRIS
BIS-TRIS (CAS: 6976-37-0; Formula: C8H19NO5; MW: 209.24) is a synthetic biological buffer belonging to the Good’s Buffers category. It exhibits good solubility in water and is recognised for its low UV and visible absorbance, making it suitable for spectrophotometric applications. This compound is particularly valued for its pKa of 6.5 at 25°C, providing effective buffering capacity within the physiological pH range of 5.8 to 7.2. It is tested for foreign activity, impurities, and suitability for cell culture and manufacturing.
- IUPAC
- 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol,2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol,Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
- Synonyms
- 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediolBis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
Biological Buffering Agent
BIS-TRIS is a versatile buffer used in biochemical and biological research. Its pKa of 6.5 ensures stable pH control for enzyme assays, protein purification, and various cell-based experiments where maintaining physiological pH is critical.
Cell Culture Media
With demonstrated suitability for cell culture, BIS-TRIS can be incorporated into media formulations to maintain optimal pH levels, supporting cell viability and growth in laboratory and manufacturing settings.
Spectrophotometric Applications
The low UV and visible light absorbance of BIS-TRIS makes it an excellent choice for buffer solutions used in spectrophotometric analyses, where interference from the buffer itself would be detrimental.
Manufacturing Processes
Designated as PharmaGrade and suitable for manufacturing use, BIS-TRIS can be employed in the production of biopharmaceuticals and other regulated products, ensuring consistent buffering throughout critical process steps.
| Molecular weight | 209.24 |
|---|---|
| Empirical formula | C8H19NO5 |
| Form | powder |
| Impurities tested | Endotoxin, microbial, and trace metals; tested |
| Suitability | suitable for cell culturesuitable for manufacturing use |
| Biological source | from synthetic |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Water hazard class (WGK, DE) | 3 |
|---|---|
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | ELITE |
|---|---|
| Product Line | PharmaGrade |
| Pka 25 °C | 6.5 |
| Foreign Activity | Cytotoxicity, DNase, NICKase, RNase, and Protease; tested |
| Useful Ph Range | 5.8 - 7.2 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is BIS-TRIS used for?
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BIS-TRIS is primarily used as a biological buffer, particularly in biochemical research, cell culture, and spectrophotometric applications. Its pKa of 6.5 makes it suitable for maintaining pH in the range of 5.8-7.2.
What are the CAS number and formula for BIS-TRIS?
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The CAS number for BIS-TRIS is 6976-37-0, and its empirical formula is C8H19NO5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies BIS-TRIS in ELITE quality, as part of our PharmaGrade product line. The product is tested for biological contaminants and foreign activity, and is suitable for cell culture and manufacturing use. We do not represent it as USP, BP, or EP grade.
What are the main safety concerns when handling BIS-TRIS?
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BIS-TRIS is classified with GHS07, carrying a Warning signal word. It poses hazards including skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335). Handle with appropriate personal protective equipment, ensuring adequate ventilation.
How is BIS-TRIS typically packed and shipped?
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BIS-TRIS is supplied as a powder. We are a specialist global supplier and exporter of fine chemicals, pharmaceutical intermediates and laboratory reagents, equipped to handle international shipments.
How can I request a quote or sample for BIS-TRIS?
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To request a quote or sample of BIS-TRIS, please contact our sales team through the details provided on our website. We are happy to assist with your specific requirements.
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