BES
BES (CAS: 10191-18-1), scientifically known as N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, is a highly pure biochemical buffer with the molecular formula C6H15NO5S. Supplied in a fine powder form, this compound is meticulously tested for biological activities, ensuring suitability for sensitive cell culture applications. Its effective buffering range and minimal UV absorption make it a reliable choice for various biochemical and pharmaceutical research processes.
- IUPAC
- N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid,N,N-Bis(2-hydroxyethyl)taurine
- Synonyms
- N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acidN,N-Bis(2-hydroxyethyl)taurine

Biochemical Research
BES serves as a critical component in biochemical studies, acting as a buffering agent to maintain stable pH conditions necessary for enzyme activity and biological reactions.
Cell Culture Media
Tested for suitability in cell culture, BES helps maintain physiological pH in growth media, supporting cell viability and experimental integrity.
Pharmaceutical Development
As a PharmaGrade buffer, it is suitable for use in pharmaceutical research and development processes where pH control is paramount.
Electrophoresis and Chromatography
Its buffering capacity and low UV absorbance make it useful in separation techniques like electrophoresis and chromatography.
| Molecular weight | 213.25 |
|---|---|
| Empirical formula | C6H15NO5S |
| Assay | ≥99.5% (T) |
| Form | powder |
| Solubility | H2O: soluble1 M at 20 °C, clear, colorless |
| pH | 2.5-5.0 (25 °C, 1 M in H2O) |
| Impurities tested | DNases, none detectedRNases, none detectedinsoluble matter, passes filter testphosphatases, none detectedproteases, none detected |
| Suitability | cell culture tested |
| Biological source | from synthetic |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Water hazard class (WGK, DE) | 3 |
|---|---|
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26 |
| Anion traces | chloride (Cl-): ≤50 mg/kgsulfate (SO42-): ≤100 mg/kg |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | ELITE |
|---|---|
| Product Line | PharmaGrade |
| Pka 25 °C | 7.1 |
| Foreign Activity | Cytotoxicity, DNase, NICKase, RNase, and Protease; tested |
| Uv Absorption | λ: 260 nm Amax: 0.095λ: 280 nm Amax: 0.080 |
| Useful Ph Range | 6.4 - 7.8 |
| Ign Residue 900 °C | ≤0.05% |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is BES used for?
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BES is primarily used as a biochemical buffer in research, particularly in cell culture media and other biological applications requiring stable pH control within its effective range of 6.4-7.8.
What is the CAS number and formula for BES?
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The CAS number for BES is 10191-18-1, and its empirical formula is C6H15NO5S.
What grade and purity does Tech Serve Solutions supply for BES?
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Tech Serve Solutions supplies BES at an ELITE quality level, with an assay of ≥99.5% (T). It is classified as PharmaGrade and is tested for biological activities such as DNase, RNase, and protease, ensuring suitability for sensitive applications. It is not represented as USP, BP, or EP grade.
How should BES be handled and stored?
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BES is classified as a warning substance, causing skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335). Handle with appropriate personal protective equipment and ensure good ventilation. Store in a cool, dry place away from incompatible materials.
Is BES available for export?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including BES, and can fulfil international orders.
How can I request a quote or sample for BES?
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To request a quote or a sample of BES, please contact our sales team through the website or by phone, providing your specific requirements.
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