bisBenzimide H 33342 trihydrochloride
bisBenzimide H 33342 trihydrochloride (CAS: 23491-52-3) is a chemical compound with the formula C27H28N6O · 3HCl · xH2O and a molecular weight of 561.93 (anhydrous basis). This fluorescent stain is primarily used for the staining of DNA, chromosomes, and nuclei. It is suitable for applications in fluorescence microscopy and flow cytometry due to its distinct excitation and emission maxima.
- IUPAC
- 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride,HOE 33342,Hoechst 33342,bisBenzimide
- Synonyms
- bisBenzimide, Hoechst 33342, HOE 33342, 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride
DNA and Chromosome Staining
This compound is an effective stain for DNA, chromosomes, and cell nuclei. Its fluorescent properties allow for clear visualisation in biological samples.
Fluorescence Microscopy
With an excitation maximum of 346 nm and emission maximum of 460 nm, bisBenzimide H 33342 trihydrochloride is well-suited for fluorescence microscopy applications, enabling the study of cellular structures.
Flow Cytometry
The compound's fluorescent characteristics make it a useful reagent for cell analysis using flow cytometry techniques.
| Molecular weight | 561.93 (anhydrous basis) |
|---|---|
| Empirical formula | C27H28N6O · 3HCl · xH2O |
| Assay | ≥98% (HPLC and TLC) |
| Solubility | H2O: soluble20 mg/mLphosphate buffer: precipitates |
| Storage temperature | −20°C |
| Application | Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.Excitation max. = 346 nmEmission max. = 460 nm |
| Suitability | suitable for fluorescence |
Hazard statements
- H302Harmful if swallowed
- H315Causes skin irritation
- H318Causes serious eye damage
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
- P310Immediately call a POISON CENTER or doctor
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-37/38 |
| Safety statements (S) | 22-36/37 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is bisBenzimide H 33342 trihydrochloride used for?
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bisBenzimide H 33342 trihydrochloride is used for staining DNA, chromosomes, and nuclei, and is suitable for fluorescence microscopy and flow cytometry.
What is the CAS number and formula for bisBenzimide H 33342 trihydrochloride?
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The CAS number is 23491-52-3 and the empirical formula is C27H28N6O · 3HCl · xH2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies bisBenzimide H 33342 trihydrochloride with an assay of ≥98% (HPLC and TLC). TSS does not represent this product as USP, BP, EP or any other pharmacopoeia grade.
What are the main safety concerns when handling bisBenzimide H 33342 trihydrochloride?
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This compound is classified as dangerous with hazards including H302 (Harmful if swallowed), H315 (Causes skin irritation), H318 (Causes serious eye damage), and H335 (May cause respiratory irritation). Appropriate personal protective equipment, including eye shields and gloves, should be worn.
How is bisBenzimide H 33342 trihydrochloride typically stored and shipped?
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The product should be stored at -20°C. Tech Serve Solutions is a global supplier and exporter of fine chemicals.
How can I request a sample or quote for bisBenzimide H 33342 trihydrochloride?
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Please contact our sales team to request a sample or a quote for bisBenzimide H 33342 trihydrochloride.
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