bisBenzimide H 33258
bisBenzimide H 33258 (CAS 23491-45-4), molecular formula C25H24N6O · 3HCl, is a high-purity chemical supplied by Tech Serve Solutions for biochemicals & reagents applications, to pharmacopoeia specification (USP, BP, EP). Every batch is tested and ships with a Certificate of Analysis; MSDS and technical data sheets are available on request. Tech Serve Solutions has supplied laboratory reagents, fine chemicals and pharmaceutical intermediates worldwide since 1998.
- IUPAC
- 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride,2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride,BBIH,BXI-72,HOE 33258,Hoechst 33258,NSC334072
| Molecular weight | 533.88 |
|---|---|
| Empirical formula | C25H24N6O · 3HCl |
| Assay | ≥98% (HPLC and TLC) |
| Grade | for fluorescence |
| Solubility | H2O: soluble10 mg/mLwater and ethanol: soluble10 mg/mL |
| Concentration | 1 mg/mL in H2O |
| Storage temperature | −20°C |
| Melting point | >300 °C(lit.) |
| Application | A bibenzimidazole dye that has been utilized as a fluorescent stain. It is membrane-permeable and selectively binds to adenine-thymine regions in the minor groove of B-form DNA 1,2 with a high binding constant, making it a useful stain for DNA, chromosomes, and nuclei 3,4. It has been shown that the fluorescence is relatively insensitive to the presence of various solution reagents (i.e., BSA, RNA, Tris, sucrose, TEA/EDTA, Triton X-100, and SDS)5. Furthermore, the solution ionic strength and pH has been shown to be independent of the Stokes’ shift of bisBenzimide H 33258 3. The properties of this dye make it useful in several applications such as detecting mycoplasma contamination in tissue culture 6,7, indicating early onset of apoptosis8,9, sorting living cells based on DNA content, and acting as a probe for DNA-Drug interactions3. Utilized in the design and |
| Suitability | passes application test for fluorescencesuitable for cell culture |
Hazard statements
- H302Harmful if swallowed
- H315Causes skin irritation
- H319Causes serious eye irritation
Precautionary statements
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-36/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Absorption | λmax 343 nm |
|---|---|
| Fluorescence | λex 355 nm; λem 465 nm in TE buffer; DNA |
Documentation
Supplied to USP, BP and EP standards. Every batch ships with a Certificate of Analysis (assay, identity, purity); MSDS and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is bisBenzimide H 33258?
+
bisBenzimide H 33258 (CAS 23491-45-4), molecular formula C25H24N6O · 3HCl, is a high-purity chemical supplied by Tech Serve Solutions for biochemicals & reagents applications, to USP, BP and EP standards.
What grades of bisBenzimide H 33258 does Tech Serve Solutions supply?
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Tech Serve Solutions supplies bisBenzimide H 33258 in USP, BP, EP and reagent/AR grades. Custom specifications for nutraceutical or specialty pharmaceutical applications are available — contact our technical sales team.
What is the CAS number and molecular formula of bisBenzimide H 33258?
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bisBenzimide H 33258 has CAS number 23491-45-4 and molecular formula C25H24N6O · 3HCl. Full specifications appear in the table above and in the Certificate of Analysis.
What is the typical minimum order quantity and lead time for bisBenzimide H 33258?
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Sample quantities are available for technical evaluation; bulk MOQ depends on grade and packaging. In-stock material typically ships in 7–10 working days; custom-spec orders take 3–4 weeks. Tech Serve Solutions ships worldwide with full export documentation.
Do you provide a Certificate of Analysis (CoA) with each shipment?
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Yes. Every batch of bisBenzimide H 33258 ships with a Certificate of Analysis covering assay, identity and purity tests, plus pharmacopoeia conformance where applicable. MSDS and TDS documents are available on request before order placement.
How should bisBenzimide H 33258 be stored?
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Store bisBenzimide H 33258 in its original sealed container in a cool, dry place away from direct sunlight and incompatible materials, in line with the storage guidance on the Safety Data Sheet. Reseal between dispensings.
What is bisBenzimide H 33258 used for?
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bisBenzimide H 33258 is used in biochemicals & reagents applications across pharmaceutical, research and industrial settings. Our technical team can advise on suitability for your specific process.
How do I request a quote for bisBenzimide H 33258?
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Request a quote with your required grade, quantity and destination via https://www.techservesolutions.in/contact or email info@techservesolutions.in. We respond with specifications, a Certificate of Analysis and pricing.
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