7-(Isobutoxycarbonyloxy)-3H-phenoxazin-3-one
7-(Isobutoxycarbonyloxy)-3H-phenoxazin-3-one (CAS: 251292-24-7, C17H15NO5) is a specialized chemical compound with a molecular weight of 313.30. It is noted for its fluorescence properties, exhibiting specific excitation and emission wavelengths after esterase cleavage. This compound is primarily utilized in biochemical and reagent applications, particularly as a fluorescent enzyme substrate and probe, enabling sensitive detection and measurement in various research contexts.
- IUPAC
- O7-(Isobutyloxycarbonyl)resorufin
- Synonyms
- O7-(Isobutyloxycarbonyl)resorufin

Fluorescent Enzyme Substrate
This compound serves as an excellent substrate for esterase enzymes. Upon enzymatic cleavage, it releases a fluorescent product, making it valuable for quantifying esterase activity in biological and biochemical assays.
Fluorescent Probe
With its characteristic fluorescence (λex 500 nm; λem ~593 nm), it functions as a fluorescent probe. This property is leveraged in various detection systems where sensitive and specific fluorescence measurements are required.
Biochemical Research Reagent
As a biochemical reagent, it is employed in a range of laboratory research settings. Its suitability for fluorescence makes it a useful tool for studying enzyme kinetics and cellular processes.
| Molecular weight | 313.30 |
|---|---|
| Empirical formula | C17H15NO5 |
| Assay | ≥97.0% (HPLC) |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 500 nm; λem ~593 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 7-(Isobutoxycarbonyloxy)-3H-phenoxazin-3-one used for?
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7-(Isobutoxycarbonyloxy)-3H-phenoxazin-3-one is primarily used as a fluorescent enzyme substrate and fluorescent probe in biochemical research, particularly for detecting esterase activity.
What is the CAS number and molecular formula?
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The CAS number is 251292-24-7 and the molecular formula is C17H15NO5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 7-(Isobutoxycarbonyloxy)-3H-phenoxazin-3-one with an assay of ≥97.0% (HPLC). TSS does not represent this product as USP, BP, or EP grade.
What are the safety precautions for handling this chemical?
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Handling requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. This compound is classified under WGK Germany 3.
How is this chemical packed, shipped, and exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, ensuring proper packaging and adherence to international shipping regulations for compounds like 7-(Isobutoxycarbonyloxy)-3H-phenoxazin-3-one.
How can I request a sample or a quote?
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Please contact the Tech Serve Solutions sales team via our website or direct inquiry channels to request a sample or a quotation for 7-(Isobutoxycarbonyloxy)-3H-phenoxazin-3-one.
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