7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester
7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester (CAS: 139346-57-9; Formula: C18H18N2O6; MW: 358.35) is a fluorescent labelling reagent. It facilitates the conjugation of the 7-(diethylamino)coumarin-3-carboxylic acid fluorophore to amine-containing molecules, such as amino acids and peptides. This ester is valuable in biochemical research for fluorescent labelling and detection applications, particularly in methods like capillary zone electrophoresis and mass spectrometry.
- IUPAC
- N-Succinimidyl 7-(diethylamino)coumarin-3-carboxylate
Fluorescent Labelling of Biomolecules
This N-succinimidyl ester is employed to covalently attach the fluorescent 7-(diethylamino)coumarin-3-carboxylic acid moiety to amine groups on biomolecules. This is crucial for visualising and quantifying molecules in biological assays and separation techniques.
Amino Acid Derivatisation for Capillary Electrophoresis
It serves as a reagent for labelling amino acids with the 7-DCCA fluorophore, enabling their sensitive detection and resolution when analysed by capillary zone electrophoresis (CZE).
Reference Standard in Mass Spectrometry
7-(Diethylamino)coumarin-3-carboxylic acid, a related compound, is used as a reference in MALDI-TOFMS for screening nerve agent degradation products. This succinimidyl ester can be used to prepare such standards or for related analytical method development.
| Molecular weight | 358.35 |
|---|---|
| Empirical formula | C18H18N2O6 |
| Assay | ≥96.0% (HPLC) |
| Solubility | DMF: solubleDMSO: solubleacetonitrile: soluble |
| Storage temperature | −20°C |
| Application | 7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester is used as a reagent to conjugate 7-(diethylamino)coumarin-3-carboxylic acid (7-DCCA) to other molecules such as amino acids via amide chemistry. 7-DCCA is used to label amino acids prior to resolution by capillary zone electrophoresis. 7-DCCA is used as a reference in screening of nerve agent degradation products by MALDI-TOFMS. |
| Suitability | corresponds for coupling to aminessuitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 445 nm; λem 482 nm in 0.1 M phosphate pH 7.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester used for?
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This compound is primarily used as a fluorescent labelling reagent to conjugate the 7-(diethylamino)coumarin-3-carboxylic acid fluorophore to amine-containing molecules, aiding in their detection and quantification in various biochemical and analytical applications.
What are the CAS number and formula for this product?
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The CAS number is 139346-57-9, and the empirical formula (Hill notation) is C18H18N2O6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥96.0% (HPLC). This is a research-grade chemical and is not supplied as USP, BP, EP, or any other pharmacopoeial standard.
What are the safety and handling precautions for this chemical?
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This compound requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or P1 (EN143) respirator filter is recommended. It carries a WGK Germany rating of 3, indicating it is hazardous to water.
How is this product shipped and exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, pharmaceutical intermediates, and laboratory reagents, equipped to handle international shipments according to regulations.
How can I request a sample or quote?
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Please contact our sales team through the website to request a sample or a detailed quote for 7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester.
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