7-Amino-4-methyl-3-coumarinacetic acid N-succinimidyl ester
7-Amino-4-methyl-3-coumarinacetic acid N-succinimidyl ester (CAS: 113721-87-2), with molecular formula C16H14N2O6 and a molecular weight of 330.29, is a blue fluorescent dye. It is reactive towards primary amine groups and is suitable for fluorescence applications. This compound finds use in FRET and LRET based studies, often employed as a donor dye in biochemical and biological research.
- IUPAC
- 7-(Dimethylamino)coumarin-4-acetic acid-NHS,7-Amino-4-methyl-3coumarinylacetyl,N-Succinimidyl 7-amino-4-methyl-3-coumarinylacetate,AMCA-NHS,Succinimidyl-7-amino-4-methylcoumarin-3-acetate
- Synonyms
- AMCA-NHS7-(Dimethylamino)coumarin-4-acetic acid-NHS7-Amino-4-methyl-3coumarinylacetylN-Succinimidyl 7-amino-4-methyl-3-coumarinylacetateSuccinimidyl-7-amino-4-methylcoumarin-3-acetate
Fluorescent Labelling
This N-succinimidyl ester derivative is designed for efficient labelling of primary amine groups present on proteins, peptides, antibodies, and other biomolecules. Its reactivity at pH 7.0-9.0 facilitates conjugation.
Fluorescence Resonance Energy Transfer (FRET)
Utilised as a donor dye in FRET studies, it can be paired with acceptors like fluorescein-5-isothiocyanate. This application leverages its fluorescent properties to study molecular interactions.
Förster Resonance Energy Transfer (LRET)
AMCA-NHS has been employed in the development of donor europium chelate fluorochromes for LRET analyses. It reacts with amino groups, enabling its integration into luminescence-based assays.
| Molecular weight | 330.29 |
|---|---|
| Empirical formula | C16H14N2O6 |
| Assay | ≥90% (HPLC) |
| Solubility | DMF: solubleDMSO: soluble |
| Storage temperature | −20°C |
| Application | AMCA-NHS has been utilized in the development of a donor europium chelate fluorochrome for use in LRET based analyses by first reacting with an amino group and then with DTPA anhydride 1,2. Utilized in FRET based studies, acting as the donor dye and can be paired with fluorescein-5-isothiocyanate as the acceptor dye 3,4,5. A blue fluorescent dye reactive towards primary amine groups on proteins, peptides, antibodies, and other biomolecules (at pH 7.0-9.0). |
| Suitability | suitable for fluorescence |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 354 nm; λem 440 nm in methanol |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 7-Amino-4-methyl-3-coumarinacetic acid N-succinimidyl ester used for?
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This compound is primarily used as a blue fluorescent dye for labelling biomolecules with primary amine groups, and in FRET and LRET based studies as a donor dye.
What are the CAS number and chemical formula?
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The CAS number is 113721-87-2, and the empirical formula is C16H14N2O6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with a minimum purity of 90% (HPLC). It is a research grade chemical and not represented as USP, BP, or EP.
How should 7-Amino-4-methyl-3-coumarinacetic acid N-succinimidyl ester be handled?
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Handle with appropriate personal protective equipment, including a dust mask type N95 (US), eyeshields, and gloves. Avoid breathing dust and ensure adequate ventilation. For GHS hazards, refer to the safety data sheet.
How is this chemical packed, shipped, and exported?
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This chemical is typically stored at -20°C. TSS is a specialist global supplier and exporter of fine chemicals, pharmaceutical intermediates, and laboratory reagents.
How can I request a sample or quote?
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Please contact our sales team via the website or phone to request a sample or a quote for 7-Amino-4-methyl-3-coumarinacetic acid N-succinimidyl ester.
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