7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester
7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester (CAS: 134471-24-2; Formula: C14H9NO7; MW: 303.22) is a reactive fluorescent labelling reagent. It is primarily used to conjugate the 7-hydroxycoumarin-3-carboxylic acid moiety to amino-containing molecules, such as amino acids and proteins, through stable amide bond formation. This ester is a valuable tool in biochemical research for developing fluorescent probes and indicators.
- IUPAC
- N-Succinimidyl 7-hydroxycoumarin-3-carboxylate,Umbelliferone-3-carboxylic acid N-succinimidyl ester
- Synonyms
- N-Succinimidyl 7-hydroxycoumarin-3-carboxylate, Umbelliferone-3-carboxylic acid N-succinimidyl ester
Fluorescent Labelling
Utilised as a reagent for the covalent attachment of the 7-hydroxycoumarin-3-carboxylic acid fluorophore to biomolecules containing primary amine groups, such as proteins and amino acids. This process enables the creation of fluorescently tagged molecules for various detection and imaging applications.
Amide Bond Formation
The N-succinimidyl ester group readily reacts with primary amines under mild conditions to form robust amide linkages. This reactivity is key to its application in conjugating the coumarin moiety to target substrates, facilitating downstream analysis or function.
Biochemical Research Reagent
Serves as a specialized reagent in biochemical and molecular biology research, contributing to the development of fluorescent probes for studying cellular processes, enzyme activity, or molecular interactions.
| Molecular weight | 303.22 |
|---|---|
| Empirical formula | C14H9NO7 |
| Assay | ≥95.0% (coupling assay to aminopropyl silica gel) |
| Solubility | DMF: solubleDMSO: soluble |
| Storage temperature | −20°C |
| Application | 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester (7-OHCCA-SE) is used as a reagent to conjugate 7-Hydroxycoumarin-3-carboxylic acid (7-OHCCA) to other molecules such as amino acids via amide chemistry. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 386 nm; λem 448 nm in 0.1 M Tris pH 9.0 |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester used for?
+
7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is used as a reagent to conjugate 7-Hydroxycoumarin-3-carboxylic acid to other molecules, such as amino acids, via amide chemistry, enabling fluorescent labelling in biochemical research.
What are the CAS number and formula for 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester?
+
The CAS number is 134471-24-2, and the empirical formula is C14H9NO7.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies this product with an assay of ≥95.0% (coupling assay to aminopropyl silica gel). It is suitable for fluorescence applications. We do not represent this product as USP, BP, or EP grade.
What are the safety considerations when handling this chemical?
+
Appropriate personal protective equipment, including eyeshields and gloves, should be worn. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The product has a WGK Germany rating of 3, indicating it is hazardous.
How is this chemical packed and shipped?
+
This chemical is typically stored at -20°C. Tech Serve Solutions specialises in the global supply and export of fine chemicals, pharmaceutical intermediates, and laboratory reagents.
How can I request a quote or sample?
+
Please contact the Tech Serve Solutions sales team to request a quote or sample. We are a specialist global supplier and exporter of fine chemicals, pharmaceutical intermediates, and laboratory reagents.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents
(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more
3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis
3-Methyl-1,5-pentanediol
Chemical Synthesis
3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis