4-Nitrophenyl phosphate magnesium salt
4-Nitrophenyl phosphate magnesium salt (CAS: 32348-90-6), with the molecular formula O2NC6H4OP(O)O2Mg and a molecular weight of 241.38 g/mol, is a valuable biochemical reagent. It serves as a chromogenic substrate for enzyme activity assays, particularly for alkaline phosphatase. Its reliable performance makes it a staple in biochemical research, diagnostics, and molecular biology laboratories for quantitative and qualitative analysis.

Enzyme Assays
This compound is primarily used as a chromogenic substrate for the determination of alkaline phosphatase activity. Upon enzymatic hydrolysis, it releases 4-nitrophenol, which can be measured spectrophotometrically.
Colorimetric Detection
It facilitates colorimetric detection methods in biochemical analysis. The intensity of the yellow colour produced by 4-nitrophenol is directly proportional to the enzyme concentration or activity.
ELISA Substrates
4-Nitrophenyl phosphate magnesium salt is employed as a substrate in Enzyme-Linked Immunosorbent Assays (ELISA) for detecting specific antigens or antibodies.
Biochemical Research
It is a useful tool in research settings for studying enzyme kinetics, characterizing enzyme inhibitors, and developing diagnostic assays.
| Molecular weight | 241.38 |
|---|---|
| Linear formula | O2NC6H4OP(O)O2Mg |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl phosphate magnesium salt used for?
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4-Nitrophenyl phosphate magnesium salt is primarily used as a chromogenic substrate for the assay of alkaline phosphatase activity and in colorimetric detection methods, including ELISA.
What are the CAS number and molecular formula for this product?
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The CAS number is 32348-90-6, and the molecular formula is O2NC6H4OP(O)O2Mg.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product as a research/technical grade chemical. We do not represent it as USP, BP, EP, or for any pharmaceutical use unless explicitly stated otherwise in the product specifications.
What are the safety and handling recommendations for this chemical?
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Handle with care, wearing appropriate personal protective equipment such as eyeshields and gloves. Use a type N95 (US) or type P1 (EN143) respirator filter. This compound has a WGK Germany rating of 3, indicating it is hazardous to water.
How is this product typically packaged and shipped?
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This chemical is typically supplied in powder form and is carefully packaged to ensure safe transit. We are a specialist global supplier and exporter of fine chemicals.
How can I request a sample or quote for 4-Nitrophenyl phosphate magnesium salt?
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Please contact our sales team through the website or by phone to request a sample or a formal quotation for 4-Nitrophenyl phosphate magnesium salt.
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