4-Nitrophenyl α-D-galactopyranoside
4-Nitrophenyl α-D-galactopyranoside (CAS: 7493-95-0; Formula: C12H15NO8) is a biochemical reagent widely employed as a substrate for enzymatic assays. Its molecular weight is 301.25 g/mol. This compound is particularly useful for the quantitative determination of α-galactosidase activity. It functions by releasing p-nitrophenol, a coloured product, upon enzymatic hydrolysis, allowing for spectrophotometric detection. It is supplied by Tech Serve Solutions for research and laboratory applications.
- IUPAC
- p-Nitrophenyl α-D-galactopyranoside
- Synonyms
- p-Nitrophenyl α-D-galactopyranoside

Enzyme Activity Assays
Utilised as a chromogenic substrate for the detection and quantification of α-galactosidase activity in biological samples. The enzymatic cleavage releases p-nitrophenol, which can be measured spectrophotometrically.
Biochemical Research
Serves as a valuable tool in biochemical research for studying enzyme kinetics and characterising α-galactosidase properties. Its use facilitates in vitro investigations into enzyme mechanisms.
Diagnostic Development
Can be employed in the development of diagnostic assays where α-galactosidase activity is a relevant biomarker. The sensitivity of the chromogenic reaction aids in early detection or monitoring.
| Molecular weight | 301.25 |
|---|---|
| Empirical formula | C12H15NO8 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl α-D-galactopyranoside used for?
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4-Nitrophenyl α-D-galactopyranoside is primarily used as a chromogenic substrate in assays to detect and quantify the activity of the enzyme α-galactosidase.
What is the CAS number and chemical formula for 4-Nitrophenyl α-D-galactopyranoside?
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The CAS number is 7493-95-0 and the chemical formula is C12H15NO8.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product for research and laboratory applications. The specific assay and purity are detailed in the product's Certificate of Analysis; it is not represented as USP, BP, EP, or pharmacopoeia grade.
How should 4-Nitrophenyl α-D-galactopyranoside be handled?
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Handle with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. It is classified under WGK Germany 3, indicating a high hazard to water.
How is 4-Nitrophenyl α-D-galactopyranoside shipped and exported?
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Tech Serve Solutions is an experienced global supplier and exporter of fine chemicals, ensuring appropriate packaging and documentation for international shipment.
How can I request a sample or quote for 4-Nitrophenyl α-D-galactopyranoside?
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Please contact our sales department through the website or by phone to request a sample or obtain a quotation for 4-Nitrophenyl α-D-galactopyranoside.
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