4-Nitrophenyl phosphate disodium salt hexahydrate
4-Nitrophenyl phosphate disodium salt hexahydrate (CAS: 333338-18-4) is a chromogenic substrate with the molecular formula O2NC6H4OP(O)(ONa)2 · 6H2O and a molecular weight of 371.14 g/mol. This compound serves as a key reagent in biochemical assays, primarily for the quantitative determination of acid and alkaline phosphatase activities. Upon enzymatic hydrolysis, it yields a soluble yellow product, enabling spectrophotometric analysis.
- IUPAC
- p-Nitrophenyl phosphate disodium hexahydrate,Disodium 4-nitrophenyl phosphate hexahydrate,di-Sodium 4-nitrophenyl phosphate,pNPP disodium hexahydrate
- Synonyms
- p-Nitrophenyl phosphate disodium hexahydrateDisodium 4-nitrophenyl phosphate hexahydratedi-Sodium 4-nitrophenyl phosphatepNPP disodium hexahydrate
Determination of Phosphatase Activity
Used as a chromogenic substrate for quantifying both acid and alkaline phosphatase levels in various biological samples. The enzymatic reaction releases p-nitrophenol, resulting in a yellow colour that can be measured spectrophotometrically at 405 nm after stopping the reaction with NaOH.
Enzyme Assay Reagent
A versatile reagent in enzyme-based diagnostic kits and research laboratories for detecting and quantifying enzyme kinetics. Its reaction product's colour intensity is directly proportional to the enzyme activity present.
Biochemical Research
Employed in fundamental biochemical studies involving enzymes that hydrolyse phosphate esters. It aids in understanding enzyme mechanisms and screening for enzyme inhibitors.
| Molecular weight | 371.14 |
|---|---|
| Linear formula | O2NC6H4OP(O)(ONa)2 · 6H2O |
| Assay | ≥97.0% (NT) |
| Solubility | H2O: soluble25 mg/mL at 20 °C, clear, yellow-green |
| Concentration | 36 - 44 mg/tablet |
| Storage temperature | 2-8°C |
| Impurities tested | ≤0.01% free nitrophenol |
| Application | Chromogenic substrate for the determination of acid and alkaline phosphatases.1,2 The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 310 nm (pH 7.1)λex 399 nm (Reaction Product) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl phosphate disodium salt hexahydrate used for?
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4-Nitrophenyl phosphate disodium salt hexahydrate is primarily used as a chromogenic substrate for the determination of acid and alkaline phosphatase activities in biochemical assays and diagnostics.
What are the CAS number and molecular formula?
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The CAS number is 333338-18-4, and the molecular formula is O2NC6H4OP(O)(ONa)2 · 6H2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥97.0% (NT). TSS does not represent this product as USP, BP, EP, or pharmacopoeia grade unless explicitly stated in the product documentation.
What are the safety and handling precautions?
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Appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter, should be used. This compound is classified under WGK Germany 3, indicating a high hazard to water.
How is this chemical supplied and exported?
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This product is typically stored at 2-8°C and is available for global export by Tech Serve Solutions, a specialist supplier since 1998.
How can I request a quote or sample?
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Please contact our sales team or use the online inquiry form on the Tech Serve Solutions website to request a quote or a sample of 4-Nitrophenyl phosphate disodium salt hexahydrate.
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