4-Nitrophenyl phosphate bis(cyclohexylammonium) salt
4-Nitrophenyl phosphate bis(cyclohexylammonium) salt (CAS: 52483-84-8) is a biochemical reagent with the molecular formula O2NC6H4OP(O)(O-)2 · 2C6H11NH3+ and a molecular weight of 417.44 g/mol. This premium quality compound serves as a chromogenic substrate for enzymatic assays, particularly for detecting alkaline phosphatase activity. It is supplied by Tech Serve Solutions for research and laboratory applications, adhering to high standards of quality and purity.
Enzyme Activity Assays
This compound is widely utilised as a chromogenic substrate for the quantitative determination of alkaline phosphatase (ALP) activity. Upon enzymatic hydrolysis, it releases 4-nitrophenol, which can be readily measured spectrophotometrically.
Colorimetric Detection
Its ability to produce a coloured product upon enzymatic cleavage makes it suitable for various colorimetric detection methods in biochemical research and diagnostics. This facilitates the identification and quantification of enzyme presence.
ELISA and Blotting Techniques
4-Nitrophenyl phosphate bis(cyclohexylammonium) salt is employed in enzyme-linked immunosorbent assays (ELISA) and blotting techniques where alkaline phosphatase is used as a reporter enzyme. It aids in the detection of specific analytes.
| Molecular weight | 417.44 |
|---|---|
| Linear formula | O2NC6H4OP(O)(O-)2 · 2C6H11NH3+ |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | PREMIUM |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt used for?
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This compound serves as a chromogenic substrate for the detection and quantification of alkaline phosphatase (ALP) activity in biochemical assays and diagnostic tests.
What are the CAS number and chemical formula?
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The CAS number is 52483-84-8 and the chemical formula is O2NC6H4OP(O)(O-)2 · 2C6H11NH3+.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product at a PREMIUM quality level. We do not represent it as USP, BP, EP, or any other pharmacopoeia grade unless explicitly stated.
What are the safety and handling considerations?
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This compound is classified with WGK Germany 3. Handle with appropriate personal protective equipment, including eyeshields and gloves, and use a type N95 (US) or type P1 (EN143) respirator filter when necessary.
How is this chemical shipped and exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, capable of shipping and exporting 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt worldwide.
How can I request a sample or quote?
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To request a sample or a quote for 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, please contact our sales department through the website or by phone.
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