4-Nitrophenyl phosphate bis(tris) salt
4-Nitrophenyl phosphate bis(tris) salt (CAS: 68189-42-4) is a chemical compound with the linear formula (C6 H4 N O6 P)(C4 H12 N O3)2 and a molecular weight of 461.36. It serves as a key chromogenic substrate for the enzymatic determination of acid and alkaline phosphatase activity. Its reliable performance makes it a valuable reagent in biochemical research and diagnostic assay development, facilitating the quantitative analysis of enzyme kinetics and expression levels.
Enzyme Substrate for Phosphatase Assays
Used as a chromogenic substrate for the quantitative determination of acid and alkaline phosphatase activity. Upon enzymatic cleavage, it releases 4-nitrophenol, which can be measured spectrophotometrically.
Biochemical Research Reagent
Valuable in various biochemical studies involving enzyme kinetics, characterization, and inhibition profiling of phosphatases. It aids in understanding enzymatic mechanisms and biological pathways.
Diagnostic Assay Development
Facilitates the development of diagnostic kits and assays for detecting phosphatase levels in biological samples. Its predictable reaction kinetics contribute to assay sensitivity and specificity.
| Molecular weight | 461.36 |
|---|---|
| Linear formula | (C6 H4 N O6 P)(C4 H12 N O3)2 |
| Assay | ≥97.0% (enzymatic) |
| Solubility | H2O: soluble0.1 g/mL, clear |
| Storage temperature | 2-8°C |
| Impurities tested | ≤0.1% free nitrophenol≤6% water |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality | for the determination of phosphatase |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl phosphate bis(tris) salt used for?
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4-Nitrophenyl phosphate bis(tris) salt is primarily used as a chromogenic substrate for the enzymatic determination of acid and alkaline phosphatase activity in biochemical research and diagnostic assay development.
What is the CAS number and formula for 4-Nitrophenyl phosphate bis(tris) salt?
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The CAS number is 68189-42-4, and its linear formula is (C6 H4 N O6 P)(C4 H12 N O3)2.
What grade and purity does Tech Serve Solutions supply for 4-Nitrophenyl phosphate bis(tris) salt?
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Tech Serve Solutions supplies 4-Nitrophenyl phosphate bis(tris) salt with an assay of ≥97.0% (enzymatic). TSS does not represent this product as USP, BP, or EP grade.
How should 4-Nitrophenyl phosphate bis(tris) salt be handled and stored safely?
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Handle with appropriate personal protective equipment, including eyeshields, gloves, and a P1 or N95 respirator filter. Store at 2-8°C. Refer to the safety data sheet for comprehensive handling guidelines. The WGK Germany classification is 3.
Is 4-Nitrophenyl phosphate bis(tris) salt available for export?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including 4-Nitrophenyl phosphate bis(tris) salt.
How can I request a quote or sample for 4-Nitrophenyl phosphate bis(tris) salt?
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Please contact our sales department via the website or phone to request a quote or sample for 4-Nitrophenyl phosphate bis(tris) salt.
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